Investigation of the pellets produced from sugarcane bagasse during liquid hot water pretreatment and their impact on the enzymatic hydrolysis

Investigation of the pellets produced from sugarcane bagasse during liquid hot water pretreatment and their impact on the enzymatic hydrolysis
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甘蔗渣在液体热水预处理过程中产生的颗粒及其对酶水解的影响的研究

DOI:
10.1016/j.biortech.2015.04.059
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发表时间:
2015
影响因子:
11.4
通讯作者:
Qi Wei
Qi Wei
中科院分区:
工程技术1区
文献类型:
--
作者:
Wang Wen;Zhuang Xinshu;Yuan Zhenhong;Yu Qiang;Qi Wei

文献摘要

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在液态热水预处理过程中,木质纤维素表面会形成大量的颗粒。研究了颗粒污泥的特性及其对低放废液处理甘蔗渣酶解的影响。在180 °C下用LHW处理SCB后,用1%氢氧化钠(NaOH)溶液温和地提取沉积在固体残余物表面上的沉淀。木质素含量为81.0%,葡聚糖含量为7.0%,木聚糖含量为3.2%。将LHW预处理溶液(PS)喷洒到滤纸上,并在其表面上观察颗粒。傅里叶变换红外光谱(FTIR)数据表明,木质素也是PS颗粒的主要成分。颗粒对酶解的影响主要是空间位阻效应,而不是纤维素酶的吸附作用。LHW处理过的SCB的结构特征可能比颗粒在影响酶解方面起更重要的作用。
In the process of liquid hot water (LHW) pretreatment, there are numbers of pellets formed on the lignocellulosic surface. The characteristics and effect of pellets on the enzymatic hydrolysis of LHW-treated sugarcane bagasse (SCB) were investigated. After SCB was treated with LHW at 180 °C, the pellets deposited on the surface of solid residues were extracted gently with 1% sodium hydroxide (NaOH) solution. They were composed of 81.0% lignin, 7.0% glucan, and 3.2% xylan. The LHW pretreatment solution (PS) was sprayed to the filter paper, and the pellets were observed on its surface. Fourier transform infrared spectroscopy (FTIR) data showed that lignin was also the main component of the PS pellets. The effect of the pellets on enzymatic hydrolysis was chiefly attributed to the steric hindrance, not the cellulase adsorption. The structural characteristics of LHW-treated SCB might play a more important role in influencing the enzymatic hydrolysis than the pellets.