Memory CD4 T-cell subsets discriminated by CD43 expression level in A-bomb survivors
Memory CD4 T-cell subsets discriminated by CD43 expression level in A-bomb survivors
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DOI：
10.3109/09553000903272641
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发表时间：
2010-01-01
影响因子：
2.6
通讯作者：
Kusunoki, Yoichiro
中科院分区：
文献类型：
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作者：
Kyoizumi, Seishi;Yamaoka, Mika;Kusunoki, Yoichiro
目的：我们以前的研究表明，辐射暴露减少了原子弹（A-炸弹）幸存者中的记忆胸腺衍生细胞（T细胞）与分化簇（CD）-4的库的多样性。为了评估原子弹幸存者在辐射暴露60年后T细胞记忆的维持情况，我们检查了幸存者外周血淋巴细胞中功能不同的记忆CD 4 T细胞亚群。方法：通过差异CD 43表达水平鉴定了三种功能不同的记忆CD 4 T细胞亚群，并使用流式细胞术进行测量。这些亚群包括功能成熟的记忆细胞、对抗原刺激反应弱的细胞和功能无能且易于自发凋亡的细胞。结果：外周血CD 4 T细胞库中这些亚群的百分比均随年龄的增长而显著增加。功能较弱和无反应性的亚群的死亡率也被发现随着辐射剂量的增加，拟合对数线性模型。然而，在记忆的CD 4 T细胞池，有辐射剂量和功能成熟的记忆cells.Conclusion的百分比之间呈负相关：这些结果表明，T细胞记忆的稳定状态，这是由细胞活化和/或细胞的生存过程中的子集，可能已被干扰的辐射照射前原子弹幸存者。
Purpose: Our previous study showed that radiation exposure reduced the diversity of repertoires of memory thymus-derived cells (T cells) with cluster of differentiation (CD)-4 among atomic-bomb (A-bomb) survivors. To evaluate the maintenance of T-cell memory within A-bomb survivors 60 years after radiation exposure, we examined functionally distinct memory CD4 T-cell subsets in the peripheral blood lymphocytes of the survivors.Methods: Three functionally different subsets of memory CD4 T cells were identified by differential CD43 expression levels and measured using flow cytometry. These subsets consist of functionally mature memory cells, cells weakly responsive to antigenic stimulation, and those cells functionally anergic and prone to spontaneous apoptosis.Results: The percentages of these subsets within the peripheral blood CD4 T-cell pool all significantly increased with age. Percentages of functionally weak and anergic subsets were also found to increase with radiation dose, fitting to a log linear model. Within the memory CD4 T-cell pool, however, there was an inverse association between radiation dose and the percentage of functionally mature memory cells.Conclusion: These results suggest that the steady state of T cell memory, which is regulated by cell activation and/or cell survival processes in subsets, may have been perturbed by prior radiation exposure among A-bomb survivors.