PAND, - NEW CHROMOSOMAL LOCUS OF SALMONELLA-TYPHIMURIUM FOR BIOSYNTHESIS OF BETA-ALANINE
PAND, - NEW CHROMOSOMAL LOCUS OF SALMONELLA-TYPHIMURIUM FOR BIOSYNTHESIS OF BETA-ALANINE
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DOI：
10.1007/bf00329783
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发表时间：
1975-01-01
期刊：
影响因子：
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通讯作者：
UBIERA, D
中科院分区：
文献类型：
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作者：
ORTEGA, MV;CARDENAS, A;UBIERA, D
用n -甲基-n′-硝基-n -亚硝基胍处理后，获得了3个鼠伤寒沙门氏菌（salmonella typhimurumlt2）突变体，它们的生长需要泛酸盐或β-丙氨酸。其表型为：SM30 Pan-、SM31 Pan- met -、SM32 Pan- thi -（对硫胺素中噻唑部分的需求）。天门冬氨酸、二氢尿嘧啶和β-尿酰丙酸均不能替代β-丙氨酸作为生长因子。通过偶联发现，3个遗传病灶（Pan-, Met-, Thi-）位于细菌染色体约128分钟处。通过转导，在Pan和Met位点之间发现63%的连锁，在Thi和Pan位点之间发现84%的连锁。噻唑类营养不良可能是由于该位点的病变所致。新的负责合成β-丙氨酸的遗传位点被命名为pand。
Three mutants ofSalmonella typhimuriumLT2, which required either pantothenate or β-alanine for growth, were obtained after treatment with N-methyl-N′-nitro-N-nitrosoguanidine. Their phenotype was: SM30 Pan-, SM31 Pan-Met-, SM32 Pan-Thi-(requirement for the thiazole-moiety of thiamine). Neither aspartate, dihydrouracil, nor β-ureidopropionate replaced β-alanine as growth factor. By conjugation it was found that the three genetic lesions (Pan-, Met-, Thi-) were located at about minute 128 of the bacterial chromosome. By transduction 63% linkage was found between the Pan and Met loci, and 84% between the Thi and Pan loci. Probably the thiazole auxotrophy was due to a lesion in thethiGlocus. The new genetic locus responsible for the synthesis of β-alanine was namedpanD.