Lysobacter lycopersici sp. nov., isolated from tomato plant Solanum lycopersicum
Lysobacter lycopersici sp. nov., isolated from tomato plant Solanum lycopersicum
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DOI：
10.1007/s10482-015-0419-1
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发表时间：
2015-03
期刊：
影响因子：
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通讯作者：
Shih-Yao Lin;A. Hameed;Cheng-Zhe Wen;You-cheng Liu;Yi-Han Hsu;Wei-An Lai;C. Young
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文献类型：
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作者：
Shih-Yao Lin;A. Hameed;Cheng-Zhe Wen;You-cheng Liu;Yi-Han Hsu;Wei-An Lai;C. Young
从切碎的番茄茎中分离到一株革兰氏阴性好氧杆状不产芽孢细菌CC-Bw-6 T。菌株CC-Bw-6 T的16 S rRNA基因序列分析表明，该菌株属于γ-变形菌纲溶胞菌属，与PanaciterraeKCTC 12600 T（97.0%）和LysobacterdaecheongensisKCTC 12600 T（96.8%）的同源性最高，与其他溶胞菌属的相似性较低（<96.5%）。菌株CC-Bw-6 T与L. PanaciterraeKCTC 12601 T的G+C含量为69.9mol%。菌株CC-Bw-6 T的主要脂肪酸为C11：0 iso、C11：0 iso 3OH、C14：0 iso、C15：0anteiso、C15：1ω 5c、C16：1ω 5c、C16：0、C15：0 iso、C16：0 iso、C17：0 iso和C16：010-甲基/C17：1 iso ω 9 c。主要极性脂质谱由磷脂酰乙醇胺、磷脂酰甘油、二磷脂酰甘油和未鉴别的磷脂组成。主要的多胺是亚精胺，泛醌-8是主要的呼吸醌。表型，基因和系统发育特征的分析表明，菌株CC-Bw-6 T相对于其他溶藻种的独特的分类地位。基于系统发育，化学分类和表型数据，我们提出了一个新的物种命名Lysobacter lycopersicisp。模式菌株为CC-Bw-6 T（=BCRC 80612 T = JCM 19164 T）。
A Gram-stain negative, aerobic, rod-shaped and non-spore-forming bacterium, CC-Bw-6Twas isolated from chopped tomato stems. Analysis of the 16S rRNA gene sequence of the strain CC-Bw-6Tshowed its affiliation with the genusLysobacterwithin the classGammaproteobacteria.Strain CC-Bw-6Twas found to be most closely related toLysobacter panaciterraeKCTC 12601T(97.0 %) andLysobacter daecheongensisKCTC 12600T(96.8 %), and showed lower similarity (<96.5 %) to otherLysobacterspecies. DNA–DNA relatedness between strain CC-Bw-6TandL. panaciterraeKCTC 12601Twas 10.8 %, the G+C content of the genomic DNA is 69.9 mol%. Strain CC-Bw-6Twas determined to possess C11:0iso, C11:0iso 3OH, C14:0iso, C15:0anteiso, C15:1ω5c, C16:1ω5c, C16:0, C15:0iso, C16:0iso, C17:0iso and C16:010-methyl/C17:1isoω9c as predominant fatty acids. The major polar lipid profile consists of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and unidentified phospholipids. The predominant polyamine is spermidine, and ubiquinone-8 is the predominant respiratory quinone. Analysis of phenotypic, geno- and phylogenetic characteristics revealed a distinct taxonomic position of strain CC-Bw-6Twith respect to otherLysobacterspecies. Based on the phylogenetic, chemotaxonomic and phenotypic data presented here, we propose a novel species with the nameLysobacter lycopersicisp. nov. The type strain is CC-Bw-6T(=BCRC 80612T= JCM 19164T).