Identification of candidate lncRNA biomarkers for renal fibrosis: A systematic review
Identification of candidate lncRNA biomarkers for renal fibrosis: A systematic review
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DOI：
10.1016/j.lfs.2020.118566
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发表时间：
2020-12-01
期刊：
影响因子：
6.1
通讯作者：
Hao, Chunsheng
中科院分区：
文献类型：
--
作者：
Jia, Hongshuai;Ma, Tiantian;Hao, Chunsheng
目的：结合LncRNA在单个肾纤维化中的表达谱研究结果，寻找潜在的LncRNA生物标志物。材料和方法：我们系统地检索了三个数据库，以确定在动物模型和人类肾纤维化中的lncRNA表达研究。从24个纳入的研究中提取了lncRNA的表达数据，并应用lncRNA计票策略来识别重要的lncRNA生物标志物。利用LncLocator算法来预测这些LncRNAs的潜在亚细胞定位。通过搜索LncBasev.2和catRAPID，获得了已识别的LncRNA生物标记物的预测靶点。关键发现：我们在731个肾纤维化啮齿动物模型和慢性肾脏病患者的样本中发现了95个差异表达的LncRNA特征，其中Tons_01181049和Tons_01496394在尿液和肾组织中普遍上调，而IncRNA-癌症易感候选2在血液和肾组织中均下调。肾纤维化动物模型中约73.33%的异常lncRNAs和CKD患者中81.82%的异常lncRNAs定位于细胞质。与这些lncRNA最相关的生物学过程和分子功能是mRNA加工和RNA结合。意义：目前的系统综述从24个研究中发现了95个显著异常的lncRNAs，未来的研究应集中在探索它们在肾脏纤维化中的潜在作用及其作为生物标志物或治疗靶点的临床应用。
Aims: To combine the results of dysregulated lncRNAs in individual renal fibrosis lncRNA expression profiling studies and to identify potential lncRNA biomarkers.Materials and methods: We systematically searched three databases to identify lncRNA expression studies of renal fibrosis in animal models and humans. The lncRNA expression data were extracted from 24 included studies, and a lncRNA vote-counting strategy was applied to identify significant lncRNA biomarkers. The lncLocator algorithm was utilized to predict the potential subcellular localization of these lncRNAs. The predicted targets of the identified lncRNA biomarkers were obtained by searching LncBase v.2 and catRAPID. Finally, GO enrichment and KEGG pathway analyses were performed.Key findings: We recognized a significant lncRNA signature of 95 differentially expressed lncRNAs in 731 samples from rodent models of renal fibrosis and CKD patients, among which TCONS_01181049 and TCONS_01496394 were commonly upregulated in both urine and renal tissues, while lncRNA-Cancer Susceptibility Candidate 2 was downregulated in both blood and renal tissues. About 73.33% dysregulated lncRNAs in renal fibrosis animal models and 81.82% dysregulated lncRNAs in CKD patients were predicted to be localized to the cytoplasm. The most relevant biological processes and molecular functions associated with these lncRNAs were mRNA processing and RNA binding.Significance: The present systematic review identified 95 significantly dysregulated lncRNAs from 24 studies and future investigations should focus on exploring their potential effects on renal fibrosis and their clinical utility as biomarkers or therapeutic targets.