Aquaporin NIP2;1 is mainly localized to the ER membrane and shows root-specific accumulation in Arabidopsis thaliana.
Aquaporin NIP2;1 is mainly localized to the ER membrane and shows root-specific accumulation in Arabidopsis thaliana.
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DOI：
10.1093/pcp/pcl004
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发表时间：
2006-10
影响因子：
4.9
通讯作者：
M. Mizutani;Satoshi Watanabe;T. Nakagawa;M. Maeshima
中科院分区：
文献类型：
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作者：
M. Mizutani;Satoshi Watanabe;T. Nakagawa;M. Maeshima
我们研究了拟南芥水通道蛋白第三亚群的一个类拟南芥素26蛋白NIP 2;1。启动子-β-葡萄糖醛酸酶融合的组织化学分析揭示了NIP 2的根特异性表达;1。NIP 2;1蛋白在幼根中检测到，但在叶、茎、花或角果中未检测到。与绿色荧光蛋白连接的NIP 2;1在拟南芥培养细胞中的瞬时表达证实了其可能的内质网定位。在酵母细胞中表达的NIP 2;1在膜中具有低的水通道活性。NIP 2;1可以用作其它小分子或离子的水通道和/或ER通道。
We investigated a nodulin 26-like protein NIP2;1, which belongs to the third subgroup of Arabidopsis aquaporins. Histochemical analysis of a promoter-beta-glucuronidase fusion revealed the root-specific expression of NIP2;1. The NIP2;1 protein was detected in young roots, but not in leaves, stems, flowers or siliques. The transient expression of NIP2;1 linked with green fluorescent protein in Arabidopsis cultured cells showed its putative endoplasmic reticulum (ER) localization. NIP2;1 expressed in yeast cells had low water channel activity in the membranes. NIP2;1 may function as a water channel and/or ER channel for other small molecules or ions.