Cross-species transmission of honey bee viruses in associated arthropods
Cross-species transmission of honey bee viruses in associated arthropods
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DOI：
10.1016/j.virusres.2013.06.013
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发表时间：
2013-09-01
期刊：
影响因子：
5
通讯作者：
Holmes, Edward C.
中科院分区：
文献类型：
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作者：
Levitt, Abby L.;Singh, Rajwinder;Holmes, Edward C.
有许多RNA病毒病原体对管理蜜蜂（Apis mellifera）的健康构成严重威胁。其中一些病毒也在更广泛的传粉者群体中被发现，这表明这些病毒在更广泛的环境传播中，对生态系统有可能产生更广泛的影响。因此，对节肢动物群落中这些病毒的生态学和进化的研究可能为这些潜在影响提供重要的见解。我们检查了管理的蜜蜂蜂群、附近的非apis膜翅目传粉昆虫和其他相关节肢动物中存在的五种常见的蜜蜂小核糖核酸样RNA病毒——黑后细胞病毒、畸形翼病毒、以色列急性麻痹病毒、克什米尔蜜蜂病毒和sacbrood病毒。值得注意的是，我们在几个节肢动物物种中观察到它们的存在。此外，利用链特异性RT-PCR检测变形翼病毒和以色列急性麻痹病毒的负链RNA表明，变形翼病毒在至少6种非apis物种中有活性复制，以色列急性麻痹病毒在1种非apis物种中有活性复制。畸形翼病毒的系统发育分析也表明该病毒在本研究中采样的物种中自由传播。总之，我们的研究表明，这些病毒不是传粉媒介群体所特有的，其他节肢动物物种也有可能参与传粉媒介群体的疾病传播。(C) 2013 Elsevier B.V.版权所有
There are a number of RNA virus pathogens that represent a serious threat to the health of managed honey bees (Apis mellifera). That some of these viruses are also found in the broader pollinator community suggests the wider environmental spread of these viruses, with the potential for a broader impact on ecosystems. Studies on the ecology and evolution of these viruses in the arthropod community as a whole may therefore provide important insights into these potential impacts. We examined managed A. mellifera colonies, nearby non-Apis hymenopteran pollinators, and other associated arthropods for the presence of five commonly occurring picorna-like RNA viruses of honey bees - black queen cell virus, deformed wing virus, Israeli acute paralysis virus, Kashmir bee virus and sacbrood virus. Notably, we observed their presence in several arthropod species. Additionally, detection of negative-strand RNA using strand-specific RT-PCR assays for deformed wing virus and Israeli acute paralysis virus suggests active replication of deformed wing virus in at least six non-Apis species and active replication of Israeli acute paralysis virus in one non-Apis species. Phylogenetic analysis of deformed wing virus also revealed that this virus is freely disseminating across the species sampled in this study. In sum, our study indicates that these viruses are not specific to the pollinator community and that other arthropod species have the potential to be involved in disease transmission in pollinator populations. (C) 2013 Elsevier B.V. All rights reserved.