Corticotropin and beta-endorphin: construction and analysis of recombinant DNA complementary to mRNA for the common precursor.
Corticotropin and beta-endorphin: construction and analysis of recombinant DNA complementary to mRNA for the common precursor.
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促肾上腺皮质激素和 β-内啡肽：与共同前体 mRNA 互补的重组 DNA 的构建和分析。
DOI：
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发表时间：
1979
影响因子：
11.1
通讯作者：
H. Goodman
中科院分区：
文献类型：
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作者：
J. Roberts;P. Seeburg;J. Shine;E. Herbert;J. Baxter;H. Goodman
由小鼠 mRNA (ACTH/LPH mRNA) 合成的 cDNA 片段，编码包含促肾上腺皮质激素 (ACTH)、β-促脂激素 (LPH) 和几种其他肽的前体多肽，已在细菌中克隆。在 cDNA 合成之前，mRNA 的 ACTH/LPH mRNA 翻译活性被富集（约 75%）。它似乎包含约 1200 个碱基，其中约 450 个碱基未翻译。克隆的DNA片段与编码蛋白质片段β-LPH-(44--90)的mRNA区域互补；它含有 [Met]-脑啡肽的所有氨基酸（β-LPH 的残基 61--65）、β-黑素细胞刺激激素的大部分氨基酸以及除 β-内啡肽的羧基端氨基酸之外的所有氨基酸。基于根据核酸序列对小鼠β-LPH的氨基酸序列进行分配，看来小鼠β-LPH与人和猪β-LPH具有广泛的同源性。该数据还建立了 β-黑素细胞刺激激素和 β-内啡肽之间作为 Lys-Arg 序列的联系。希望这个克隆的DNA可以作为探针来研究ACTH/LPH基因的表达和结构。
A cDNA fragment synthesized from mouse mRNA (ACTH/LPH mRNA) that codes for the precursor polypeptide containing corticotropin (ACTH), beta-lipotropin (LPH), and several other peptides has been cloned in bacteria. The mRNA was enriched for ACTH/LPH mRNA translational activity (to about 75%) prior to cDNA synthesis. It appears to contain about 1200 bases, of which approximately 450 bases are not translated. The cloned DNA fragment is complementary to the region of the mRNA coding for the protein fragment beta-LPH-(44--90); this contains all of the amino acids of [Met]-enkephalin (residues 61--65 of beta-LPH), most of the amino acids of beta-melanocyte-stimulating hormone, and all but the carboxy-terminal amino acid of beta-endorphin. Based on assignment of the amino acid sequence of mouse beta-LPH from the nucelic acid sequence, it appears that there is extensive homology of mouse beta-LPH with human and porcine beta-LPH. The data also establish the linkage between beta-melanocyte-stimulating hormone and beta-endorphin as a Lys-Arg sequence. It is hoped that this cloned DNA can be used as a probe to study the expression and structure of the ACTH/LPH gene.