Trichoplusia ni granulosis virus granulin: a phenol-soluble, phosphorylated protein
Trichoplusia ni granulosis virus granulin: a phenol-soluble, phosphorylated protein
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DOI：
10.1128/jvi.16.5.1108-1116.1975
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发表时间：
1975-11
影响因子：
5.4
通讯作者：
M. Summers;G. Smith
中科院分区：
文献类型：
--
作者：
M. Summers;G. Smith
粉纹夜蛾颗粒病毒颗粒蛋白由一种主要多肽成分组成，估计分子量为 28,000。该蛋白质可溶于酚、磷酸化且呈酸性。碱性条件激活的蛋白酶也与溶解的颗粒蛋白制剂有关。如果未正确灭活，蛋白酶将在蛋白质中引入大量伪影，导致 SDS 聚丙烯酰胺凝胶电泳和肽图谱分析结果不明确且不正确。记录了酶失活和制备高度纯化形式的颗粒蛋白以进行表征的程序。
Trichoplusia ni granulosis virus granulin consists of one major polypeptide component with an estimated molecular weight of 28,000. The protein is phenol soluble, phosphorylated, and acidic. A protease activated by alkaline conditions is also associated with solubilized granulin preparations. If not properly inactivated, the protease will introduce extensive artifact into the protein giving rise to ambiguous and incorrect results as analyzed by SDS-polyacrylamide gel electrophoresis and peptide mapping. Procedures are documented for enzyme inactivation and the preparation of granulin in highly purified form for characterization.