Annotation and structural elucidation of bovine milk oligosaccharides and determination of novel fucosylated structures
Annotation and structural elucidation of bovine milk oligosaccharides and determination of novel fucosylated structures
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DOI：
10.1093/glycob/cwt007
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发表时间：
2013-06-01
期刊：
影响因子：
4.3
通讯作者：
Barile, Daniela
中科院分区：
文献类型：
--
作者：
Aldredge, Danielle L.;Geronimo, Maria R.;Barile, Daniela
牛乳低聚糖(BMO)被乳制品和食品行业以及婴儿配方奶粉制造商公认为新型、高潜力的生物活性食品配料。最近的研究表明，牛乳含有复杂的低聚糖，在结构上与以前认为只存在于人奶中的低聚糖有关。这些BMO是参与重要生物活性的微生物调节剂，包括防止病原体与肠道上皮结合，并作为选定类别有益细菌的营养物质。只有少量的BMO结构被完全阐明。为了更好地了解BMO作为一类生物治疗药物的潜力，需要对其进行详细的结构分析。这项研究启动了BMOS结构文库的开发和结构相关特异性的综合评估。用高效质谱仪和先进的分离技术对牛乳中的糖类进行了分析，得到了一个全面的BMO目录，其中包括几种新的、丰度较低的中性寡糖和岩藻糖化寡糖，这些寡糖在分析过程中经常被忽视。使用异构体特定串联质谱仪和靶向外切糖苷酶消化鉴定结构，以产生详细说明保留时间、准确质量和结构的BMO文库，以便在未来的研究中快速鉴定它们。
Bovine milk oligosaccharides (BMOs) are recognized by the dairy and food industries, as well as by infant formula manufacturers, as novel, high-potential bioactive food ingredients. Recent studies revealed that bovine milk contains complex oligosaccharides structurally related to those previously thought to be present in only human milk. These BMOs are microbiotic modulators involved in important biological activities, including preventing pathogen binding to the intestinal epithelium and serving as nutrients for a selected class of beneficial bacteria. Only a small number of BMO structures are fully elucidated. To better understand the potential of BMOs as a class of biotherapeutics, their detailed structure analysis is needed. This study initiated the development of a structure library of BMOs and a comprehensive evaluation of structure-related specificity. The bovine milk glycome was profiled by high-performance mass spectrometry and advanced separation techniques to obtain a comprehensive catalog of BMOs, including several novel, lower abundant neutral and fucosylated oligosaccharides that are often overlooked during analysis. Structures were identified using isomer-specific tandem mass spectroscopy and targeted exoglycosidase digestions to produce a BMO library detailing retention time, accurate mass and structure to allow their rapid identification in future studies.