GoldNanocatalystsTowardsDigitalSensingProbeswithElectrochemiluminescenceBasedMicroElectrodesArray
GoldNanocatalystsTowardsDigitalSensingProbeswithElectrochemiluminescenceBasedMicroElectrodesArray
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金纳米催化剂迈向基于电化学发光的微电极阵列数字传感探针
DOI：
10.1002/elan.202100312
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发表时间：
2022
期刊：
影响因子：
3
通讯作者：
Kohei Nagano,Yuhei Terada,Akiko Araki,Shuto Osaki,Masato Saito,Eiichi Tamiya
中科院分区：
文献类型：
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作者：
森笹瑞季;木村圭佑;水重貴文;蕪山由己人;内田健志;吉田恵里子;森司;井上菜穂子;Kohei Nagano,Yuhei Terada,Akiko Araki,Shuto Osaki,Masato Saito,Eiichi Tamiya
作者发现，金纳米颗粒（AuNP）作为纳米催化剂，用于从溶解氧中产生活性氧物质，如过氧化氢，与三（羟甲基）氨基甲烷（Tris）或N-[三（羟甲基）甲基]甘氨酸（Tricine）化学品共存，并且这些可以通过鲁米诺电化学发光（ECL）进行评估。在本研究中，我们研究了反应时间，颗粒浓度和温度对金纳米粒子的催化性能的影响。与酶等生物催化剂不同，金纳米颗粒显示出阿克里尼乌斯图样的活性随温度的增加而没有失活。此外，对于不同粒径（5、15、30、50和400 nm）的球形金纳米粒子，金纳米粒子的催化活性随其粒径和形状的不同而不同，且每个金纳米粒子的催化活性随粒径和表面积的增加而增加。 当比较单位表面积的活性时，15 nm的纳米颗粒显示出更高的活性。 我们制备了纳米花（AuNF）结构的金纳米粒子的大小约。200 nm，表现出更高的活性比400 nm的球形颗粒。 表面积的增加和表面条件的差异可能会影响这些结果。我们还制备了能够容纳少量nl-pL测试试剂的微电极阵列。他们展示了在数十个纳米颗粒的水平上对金纳米颗粒的活性进行ECL成像的可能性，这可能导致纳米催化剂作为数字分析的探针。
The authors have found that gold nanoparticles (AuNP) function as nanocatalysts for the generation of reactive oxygen species such as hydrogen peroxide from dissolved oxygen in coexistence with tris(hydroxymethyl)aminomethane (Tris) orN‐[tris(hydroxymethyl) methyl]glycine (Tricine) chemicals, and that these can be evaluated by luminol electrochemi‐luminescence (ECL). In the present study, we investigated the effects of reaction time, particle concentration, and temperature on the catalytic properties of gold nanoparticles. Unlike biocatalysts such as enzymes, the gold nanoparticles showed an Arrhenius plot‐like increase in activity with temperature without inactivation. In addition, the catalytic activity of the gold nanoparticles varied with the size and shape of the nanoparticles for spherical gold nanoparticles of different sizes (5, 15, 30, 50 and 400 nm) and the activity per nanoparticle increased with the size and surface area. When compared activity per surface area, 15 nm nanoparticles showed higher activity. We prepared gold nanoparticles with nanoflower (AuNF) structure of approx.200 nm in size which showed higher activity per particle than 400 nm spherical particles. The increase in surface area and the difference in surface condition may have influenced these results. We also prepared microelectrode arrays capable of holding small amounts of nl‐pL test reagents. They showed the possibility of imaging ECL of the activity of gold nanoparticles at the level of tens of nanoparticles which could lead to nanocatalysts as probes for digital analysis.