Chondromodulin-I and tenomodulin are differentially expressed in the avascular mesenchyme during mouse and chick development
Chondromodulin-I and tenomodulin are differentially expressed in the avascular mesenchyme during mouse and chick development
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DOI：
10.1007/s00441-007-0570-8
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发表时间：
2008-04-01
影响因子：
3.6
通讯作者：
Hiraki, Yuji
中科院分区：
文献类型：
--
作者：
Shukunami, Chisa;Takimoto, Aki;Hiraki, Yuji
软骨调节素-I（ChM-I）和腱调节素（TeM）是同源的血管生成抑制剂。我们分析了毛细血管网络和这些分子在小鼠和鸡发育过程中的定位之间的空间关系。ChM-I和TeM蛋白定位于PECAM-1阴性无血管区域：ChM-I在无血管软骨中表达，而TeM在致密结缔组织（包括肌腱和韧带）中可检测到。我们还检查了血管的鸡胚注射印度墨水，并进行了原位杂交的ChM-Ⅰ和TeM基因。ChM-I表达的开始与小鼠胚胎发育期间的软骨形成相关。ChM-I表达也可在鸡胚的前软骨或非软骨无血管间充质中检测到，包括体节、硬节和心脏。因此，在脊椎动物发育过程中，ChM-I和TeM的表达结构域包含间充质组织的典型无血管区域。
Chondromodulin-I (ChM-I) and tenomodulin (TeM) are homologous angiogenesis inhibitors. We have analyzed the spatial relationships between capillary networks and the localization of these molecules during mouse and chick development. ChM-I and TeM proteins have been localized to the PECAM-1-negative avascular region: ChM-I is expressed in the avascular cartilage, whereas TeM is detectable in dense connective tissues, including tendons and ligaments. We have also examined the vasculature of chick embryos by injection with India ink and have performed in situ hybridization of the ChM-I and TeM genes. The onset of ChM-I expression is associated with chondrogenesis during mouse embryonic development. ChM-I expression is also detectable in precartilaginous or noncartilaginous avascular mesenchyme in chick embryos, including the somite, sclerotome, and heart. Hence, the expression domains of ChM-I and TeM during vertebrate development incorporate the typical avascular regions of the mesenchymal tissues.