Role for p300 in Pax 8 induction of thyroperoxidase gene expression
Role for p300 in Pax 8 induction of thyroperoxidase gene expression
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DOI：
10.1074/jbc.m003043200
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发表时间：
2000-11-03
影响因子：
4.8
通讯作者：
Civitareale, D
中科院分区：
文献类型：
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作者：
De Leo, R;Miccadei, S;Civitareale, D
核p300蛋白作为基因转录的辅助激活因子发挥作用。在这里，我们证明了p300作为转录因子Pax 8在甲状腺过氧化物酶基因启动子上的激活作用。与其作为共激活因子的作用一致，p300增强了Pax 8激活的转录。此外，我们提供了支持在体外和体外两种因素之间形成复合体的证据。这种相互作用涉及p300的氨基末端和CH3结构域以及Pax 8的羧基末端的反式激活结构域。我们发现CH3结构域对于p300在甲状腺过氧化物酶基因启动子上的共同激活作用是至关重要的。与我们的发现和腺病毒蛋白E1a结合p300的能力一致，我们发现EIA下调了Pax 8的活性。
The nuclear p300 protein functions as a co-activator of gene transcription. Here we show that p300 works as a ca-activator of the transcription factor Pax 8 on the thyroperoxidase gene promoter. Consistent with its role as co-activator, p300 potentiates Pax 8-activated transcription. Furthermore, we provide evidence supporting the formation of a complex between both factors in vitro and in vitro. This interaction involves the aminoterminal and CH3 domains of p300 and the trans-activation domain of Pax 8 at its carboxyl-terminal end. We show that the CH3 domain is crucial for the co-activator role of p300 on the thyroperoxidase gene promoter. In agreement with our finding and with the ability of the adenoviral protein E1A to bind p300, we show that EIA down-regulates Pax 8 activity.