COPPER DEFICIENCY INCREASES IN-VIVO HEPATIC SYNTHESIS OF FATTY-ACIDS, TRIACYLGLYCEROLS, AND PHOSPHOLIPIDS IN RATS
COPPER DEFICIENCY INCREASES IN-VIVO HEPATIC SYNTHESIS OF FATTY-ACIDS, TRIACYLGLYCEROLS, AND PHOSPHOLIPIDS IN RATS
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DOI：
10.3181/00379727-204-43640
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发表时间：
1993-10-01
影响因子：
--
通讯作者：
LEI, KY
中科院分区：
文献类型：
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作者：
ALOTHMAN, AA;ROSENSTEIN, F;LEI, KY
本研究探讨了饮食铜的影响，在体内肝脂肪酸的合成和新生脂肪酸纳入各种肝脏脂质类。将50只刚断奶的雄性Sprague-Dawley大鼠分配到两种饮食处理组，铜缺乏（5.4 nmol/g饮食）和铜充足（102 nmol/g饮食）。处理7周后，通过股静脉向大鼠注射0.111 MBq [1-C-14]乙酸盐（1.85 GBq/mM）/100 g体重。在注射后3、6、9、12和24 min处死各处理组的5只大鼠。新生脂肪酸样品的放射性用于测定脂肪酸合成及其组装成三酰甘油和磷脂的相对速率。两种治疗的总肝脂肪酸合成及其组装成三酰甘油和磷脂在注射后12 min内均观察到线性增加。此外，46%和30%的总脂肪酸合成组装成三酰甘油和磷脂，分别为两组。此外，肝脂肪酸合成和组装成三酰甘油和磷脂增强2倍以上的铜缺乏时，数据表示为每肝脏每100克体重。
This study examined the influence of dietary copper status on the in vivo hepatic fatty acid synthesis and the incorporation of nascent fatty acids into various hepatic lipid classes. Fifty weanling male Sprague-Dawley rats were assigned to two dietary treatments, copper deficient (5.4 nmol/g of diet) and copper adequate (102 nmol/g of diet). After 7 weeks of treatment, rats were injected with 0.111 MBq of [1-C-14] acetate (1.85 GBq/mM)/100 g body wt through the femoral vein. Five rats from each treatments were sacrificed at 3, 6, 9, 12, and 24 min after injection. Radioactivities of nascent fatty acid samples were used to determine relative rates of fatty acid synthesis and their assembly into triacylglycerols and phospholipids. Linear increases were observed up to 12 min after injection for total hepatic fatty acid synthesis and their assembly into triacylglycerols and phospholipids for both treatments. In addition, 46% and 30% of total fatty acid synthesized were assembled into triacylglycerols and phospholipids, respectively, for both groups. Furthermore, hepatic fatty acid synthesis and assembly into triacylglycerols and phospholipids were enhanced more than 2-fold by copper deficiency when the data were expressed as per liver per 100 g body weight.