2 TANDEMLY ORGANIZED HUMAN GENES ENCODING THE T-CELL GAMMA CONSTANT-REGION SEQUENCES SHOW MULTIPLE REARRANGEMENT IN DIFFERENT T-CELL TYPES
2 TANDEMLY ORGANIZED HUMAN GENES ENCODING THE T-CELL GAMMA CONSTANT-REGION SEQUENCES SHOW MULTIPLE REARRANGEMENT IN DIFFERENT T-CELL TYPES
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DOI：
10.1038/316464a0
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发表时间：
1985-01-01
期刊：
影响因子：
64.8
通讯作者：
RABBITTS, TH
中科院分区：
文献类型：
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作者：
LEFRANC, MP;RABBITTS, TH
最近对T细胞中重排基因的详细分析表明，编码α和β链的T细胞受体基因与免疫球蛋白基因类似，参与了T细胞DNA的特异性改变。从小鼠细胞毒性T淋巴细胞中分离出了第三类基因γ，有证据表明，小鼠在该基因系统中表现出非常有限的多样性，只有三个可变区(V)基因和三个恒定区(C)基因。所谓的t细胞γ基因的功能尚不清楚。我们已经分离出编码人类同源的小鼠t细胞γ基因的基因组基因；由于没有证据表明这个t细胞重排基因与T3分子有任何关系，我们将人类t细胞重排基因命名为TRGγ（参考文献）。13)，以避免与t3 γ-链混淆，并表明该基因位点在人类的7号染色体上。我们现在报道，人类DNA包含两个串联排列的trg γ恒定区基因，相隔约16千碱基。这两个基因在多种T细胞中表现出多种重排模式，包括辅助型和细胞毒性/抑制型，以及所有形式的T细胞白血病。我们的结果表明，与小鼠的类似系统相比，人类的这种t细胞基因系统具有可变性。
The recent detailed analysis of genes that undergo rearrangement in T cells has shown that the T-cell receptor genes encodingαandβ-chains are involved in specific alterations in T-cell DNA analogous to the immunoglobulin genes1–9. A third type of gene, designatedγ, has been isolated from mouse cytotoxic T lymphocytes10, and evidence suggests that the mouse displays very limited diversity in this gene system11, having only three variable-region (V) genes and three constant-region (C) genes12. The function of the so-called T-cellγgene is unknown. We have isolated genomic genes encoding the human homologue of the mouse T-cellγgene; as there is no evidence that this T-cell rearranging gene is anything to do with the T3 molecule, we have designated the human T-cell rearranging gene as TRGγ(ref. 13), to avoid confusion with the T3γ-chain, and have shown that the gene locus maps to chromosome 7 in humans13. We now report that human DNA contains two tandemly arranged TRGγconstant-region genes about 16 kilobases apart. These two genes show multiple rearrangement patterns in a variety of T cells, including helper and cytotoxic/suppressor type, as well as in all forms of T-cell leukaemia. Our results indicate variability of this T-cell gene system in man compared with the analogous system in mouse.