The biosynthesis and processing of vitellogenin in the fat bodies of females and males of the cockroach Leucophaea maderae
The biosynthesis and processing of vitellogenin in the fat bodies of females and males of the cockroach Leucophaea maderae
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DOI：
10.1016/s0965-1748(97)00071-4
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发表时间：
1997-11-01
影响因子：
3.8
通讯作者：
Engelmann, F
中科院分区：
文献类型：
--
作者：
Don-Wheeler, G;Engelmann, F
用保幼激素类似物(JHA)甲基丁二烯诱导白纹伊蚊成虫和雄虫卵黄蛋白前体卵黄蛋白原(VG)的合成。雌性和雄性产生的卵黄原蛋白(VGF和VGM)分别含有112、95、92和54 kDa的多肽，分泌的卵黄原蛋白中还存在少量的短暂的155 kDa的过渡性VG多肽和数量可变的85 kDa的物种。定量上，VGF和VGM在Vg112和Vg95单位(VGF&Gt；VGM)和Vg85多肽(VGF<；在本研究中，用S-35-蛋氨酸/半胱氨酸和P-32-正磷酸盐组成的短脉冲电脉冲，研究了雌性和雄性脂肪体中VG前体的生物合成。通过内切糖苷酶H消化和衣霉素抑制N-连接糖基化来检测VG前体的糖基化。结果表明，在雌性和雄性中，卵黄蛋白原前体的合成是分步骤进行的：(1)Vg203的共翻译糖基化；(2)Vg203翻译后的磷酸化形成Vg220；(3)Vg220的蛋白降解过程，形成组成Vg的多肽。女性和男性的203和220 kDa的VG前体似乎被类似的糖基化和磷酸化，Vg112到Vg85的额外加工在男性的脂肪体中比女性更明显，似乎解释了这些多肽在VGF和VGM中分布的数量差异，最后，VGF和VGM的主要寡糖似乎是那些N-连接甘露糖残基的寡糖，用衣霉素治疗女性和男性表明VG前体的共翻译糖基化是VG的磷酸化所必需的，以及VG前体从脂肪体分泌，(C)1998 Elsevier Science Ltd.保留所有权利。
The juvenile hormone analog (JHA) methoprene was used to induce the synthesis of the yolk protein precursor vitellogenin (Vg) in adult females and;males of the cockroach Leucophaea maderae. The female-and male-produced vitellogenin !(VgF and VgM, respectively) contained polypeptides of 112, 95, 92, and 54 kDa, Also present in the secreted vitellogenins was a small quantity of a short-lived transitional 155 kDa Vg polypeptide, and a variable amount of an 85 kDa species, Quantitatively, the VgF and VgM were significantly different in the Vg112 and Vg95 units (VgF > VgM), and in the Vg85 polypeptide (VgF < VgM), In the present study, the biosynthesis of Vg precursors in the fat bodies of females and males was examined using a short radiopulse with S-35-methionine/cysteine and P-32-orthophosphate. The glycosylation of the Vg precursors was examined by digestion with endoglycosidase H and by the inhibition of N-linked glycosylation with tunicamycin. The data showed that in both females and males, the synthesis of the vitellogenin precursor occurred in a stepwise fashion: (1) the co-translational glycosylation of Vg203; (2) the post-translational phosphorylation of Vg203 to form Vg220; (3) the proteolytic processing of Vg220 to form the constituent Vg polypeptides. The 203 and 220 kDa Vg precursors of females and males appeared to be similarly glycosylated and phosphorylated, The additional processing of Vg112 to Vg85 was more pronounced in the fat bodies of males than in females, and appears to account for the quantitative difference in the distribution of these polypeptides in VgF and VgM, Finally, the major oligosaccharides of VgF and VgM appear to be those of N-linked mannose residues, The treatment of females and males with tunicamycin indicated that the co-translational glycosylation of Vg precursors was required for the phosphorylation of the Vg precursor, as well as the secretion of Vg from the fat body, (C) 1998 Elsevier Science Ltd. All rights reserved.