Relative ability of modified versions of the hamster oocyte penetration test, incorporating hyperosmotic medium or the ionophore A23187, to predict IVF outcome.
Relative ability of modified versions of the hamster oocyte penetration test, incorporating hyperosmotic medium or the ionophore A23187, to predict IVF outcome.
复制标题
修改版仓鼠卵母细胞渗透测试（结合高渗介质或离子载体 A23187）预测 IVF 结果的相对能力。
作者：
R. Aitken;S. Thatcher;A. Glasier;J. S. Clarkson;F. Wu;D. Baird
本研究旨在评估IVF结局与两种改良版本的无透明带仓鼠卵母细胞穿透试验结果之间的关系，其中精子与高渗培养基或二价阳离子离子载体A23187预孵育。当使用前一种系统时，观察到与IVF结局的相关性较差。在IVF之前筛选的样本显示出60%的假阴性率（渗透试验失败，IVF成功），而对于那些与IVF同时评估的样本，等效数字为85.7%。添加A23187优化了穿透系统，使精子-卵母细胞融合水平更高，并能更准确地预测精子体外与人卵子受精的能力。使用该系统，筛选样本的假阴性率为4.3%，与IVF同时评估的假阴性率为0%。这些结果表明，A23187增强系统可能是一个有价值的体外受精的筛选标准。
This study was designed to assess the relationship between IVF outcome and the results obtained with two modified versions of the zona-free hamster oocyte penetration test in which the spermatozoa were pre-incubated with either hyperosmotic medium or the divalent cation ionophore A23187. When the former system was used, a poor correlation with IVF outcome was observed. Samples screened prior to IVF exhibited a 60% false negative rate (failed penetration test, successful IVF), while for those assessed concurrently with IVF, the equivalent figure was 85.7%. Addition of A23187 optimized the penetration system giving higher levels of sperm--oocyte fusion and a more accurate prediction of the capacity of the spermatozoa to fertilize human ova in vitro. With this system the false negative rate was 4.3% for screened samples and 0% for those assessed simultaneously with IVF. These results suggest that the A23187-enhanced system may be of value as a screening criterion for IVF.