NMDA receptor-dependent processes in the medial prefrontal cortex are important for acquisition and the early stage of consolidation during trace, but not delay eyeblink conditioning
NMDA receptor-dependent processes in the medial prefrontal cortex are important for acquisition and the early stage of consolidation during trace, but not delay eyeblink conditioning
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DOI：
10.1101/lm.5905
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发表时间：
2005-11-01
影响因子：
2
通讯作者：
Kirino, Y
中科院分区：
文献类型：
--
作者：
Takehara-Nishiuchi, K;Kawahara, S;Kirino, Y
内侧前额叶皮质(MPFC)的永久性损伤会影响对条件反应(CRS)的习得和远程习得CRS的保留。为了进一步阐明mPFC在这类学习中的作用，我们通过向大鼠mPFC局部微量注射GABA(A)受体激动剂Muscimol或NMDA受体拮抗剂APV，研究了mPFC在日常条件反射过程中和之后对助记过程的参与。如果在充分的学习后使用，在每日条件反射前向mPFC内注射蝇草醇可显著延缓CR的获得，并减少CR的表达。APV输注也会损害CRS的获得性，但不会损害习得性CRS的表达。当每日条件化后立即输液时，蝇草醇组和APV输注组的CR获得均受到部分损害。相比之下，每日条件反射后3小时注射蝇草酚的大鼠，其CR表现与对照组相当。在延迟范式中，预先和后处理灌输蝇毒酚对习得均无影响。这些结果表明，mPFC参与了NMDA受体介导的瞬目条件反射的获得和痕迹记忆巩固的早期阶段，但不延迟眨眼条件反射。
Permanent lesions in the medial prefrontal cortex (mPFC) affect acquisition of conditioned responses (CRs) during trace eyeblink conditioning and retention of remotely acquired CRs. To clarify further roles of the mPFC in this type of learning, we investigated the participation of the mPFC in mnemonic processes both during and after daily conditioning using local microinfusion of the GABA(A) receptor agonist muscimol or the NMDA receptor antagonist APV into the rat mPFC. Muscimol infusions into the mPFC before daily conditioning significantly retarded CR acquisition and reduced CR expression if applied after sufficient learning. APV infusion also impaired acquisition of CRs, but not expression of well-learned CRs. When infusions were made immediately after daily conditioning, acquisition of the CR was partially impaired in both the muscimol and APV infusion groups. In contrast, rats that received muscimol infusions 3 h after daily conditioning exhibited improvement in their CR performance comparable to that of the control group. Both the pre- and post-conditioning infusion of muscimol had no effect on acquisition in the delay paradigm. These results suggest that the mPFC participates in both acquisition of a CR and the early stage of consolidation of memory in trace, but not delay eyeblink conditioning by NMDA receptor-mediated operations.