DNA REARRANGEMENTS IN ORGAN-SPECIFIC VARIANTS OF POLYOMAVIRUS JC STRAIN GS
DNA REARRANGEMENTS IN ORGAN-SPECIFIC VARIANTS OF POLYOMAVIRUS JC STRAIN GS
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DOI：
10.1128/jvi.62.5.1730-1735.1988
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发表时间：
1988-05-01
影响因子：
5.4
通讯作者：
DORRIES, K
中科院分区：
文献类型：
--
作者：
LOEBER, G;DORRIES, K
JC 病毒 (JCV) GS 株的变种是直接从进行性多灶性白质脑病患者的中枢神经系统（变种 GS/B）和肾脏（变种 GS/K）中分离出来的，并进行了克隆和测序。分离株的基因组在其蛋白质编码区的核苷酸序列上几乎相同，这表明两者都起源于单一感染性 JCV 基因组。相比之下，假定的转录控制元件的排列显示出相当大的差异。在 JCV GS/B 变体的增强子区域内发现两次的串联重复元件在 GS/K 变体中不存在。缺失的元件被含有猿猴病毒 40 和腺病毒 E1A 核心增强子元件的 DNA 片段取代。器官特异性 GS 变体的这些差异表明，转录控制元件内的重排可能与 JCV 感染过程中病毒与细胞相互作用的改变有关。
Variants of JC virus (JCV) strain GS were isolated directly from the central nervous system (variant GS/B) and the kidney (variant GS/K) of a patient with progressive multifocal leukoencephalopathy and were cloned and sequenced. The genomes of the isolates were shown to be nearly identical in the nucleotide sequences of their protein-coding regions, suggesting that both had originated from a single infecting JCV genome. In contrast, the arrangement of the putative elements of transcriptional control revealed considerable differences. The tandemly repeated elements found twice within the enhancer region of JCV GS/B variant were not present in the GS/K variant. The missing elements were replaced by DNA segments containing simian virus 40 and adenovirus E1A core enhancer elements. These differences in the organ-specific GS variants suggest that rearrangements within elements of transcriptional control might be involved in altering the virus-cell interaction in the course of a JCV infection.