Suppression of SlNAC1 reduces heat resistance in tomato plants
Suppression of SlNAC1 reduces heat resistance in tomato plants
复制标题
SlNAC1 的抑制会降低番茄植株的耐热性
DOI：
10.1007/s10535-014-0477-7
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发表时间：
2015-01-01
影响因子：
1.5
通讯作者：
Meng, Q. -W.
中科院分区：
文献类型：
--
作者：
Liang, X. -Q.;Ma, N. -N.;Meng, Q. -W.
NAC（NAM、ATAF 1、2和CUC 2）转录因子在植物对各种环境胁迫的响应中起重要作用。研究番茄（Solanum lycopersicum L.）中ATAF亚家族成员SlNAC 1的功能。植物在热胁迫条件下，使用反义技术产生转基因番茄植物。在40 °C下处理48 h后，与野生型（WT）植物相比，转基因植物严重萎蔫，并且表现出较低的净光合速率和最大的光系统II光化学效率。此外，转基因植株表现出较高的离子渗漏率和丙二醛含量以及较低的脯氨酸含量。转基因植株体内活性氧（超氧阴离子自由基和过氧化氢）含量高于野生型植株，抗坏血酸过氧化物酶和超氧化物歧化酶活性低于野生型植株。转基因植株还表现出一些热休克蛋白基因（Hsp70、Hsp90、sHsp17.4和sHsp17.6）的转录本的较低积累。这些结果表明，抑制SlNAC 1基因表达可明显降低番茄的耐热性，表明SlNAC 1基因在番茄耐热性中起重要作用。
NAC (NAM, ATAF1,2, and CUC2) transcription factors play an important role in the responses of plants to various environmental stresses. To investigate the function of SlNAC1, which was found to be a member of the ATAF subfamily in tomato (Solanum lycopersicum L.) plants under heat stress conditions, transgenic tomato plants were generated using an antisense technology. After a treatment at 40 °C for 48 h, in comparison with wild-type (WT) plants, the transgenic plants were severely wilted and exhibited a lower net photosynthetic rate and a maximal photochemical efficiency of photosystem II. Moreover, the transgenic plants displayed a higher ion leakage and malondialdehyde content and a lower proline content. The content of reactive oxygen species (superoxide anion radicals and hydrogen peroxide) were higher, and activities of ascorbate peroxidase and superoxide dismutase lower in the transgenic plants than in the WT plants. The transgenic plants also exhibited a lower accumulation of the transcripts of some heat shock protein genes (Hsp70, Hsp90, sHsp17.4, and sHsp17.6). All of these results suggest that the suppression of SlNAC1 could obviously reduce heat resistance in the tomato plants, and this indicates that SlNAC1 played an important role in the thermal tolerance of the tomato plants.