HAEMOPHILUS-INFLUENZAE RESIDES AND MULTIPLIES INTRACELLULARLY IN HUMAN ADENOID TISSUE AS DEMONSTRATED BY IN-SITU HYBRIDIZATION AND BACTERIAL VIABILITY ASSAY
HAEMOPHILUS-INFLUENZAE RESIDES AND MULTIPLIES INTRACELLULARLY IN HUMAN ADENOID TISSUE AS DEMONSTRATED BY IN-SITU HYBRIDIZATION AND BACTERIAL VIABILITY ASSAY
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DOI：
10.1128/iai.62.2.673-679.1994
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发表时间：
1994-02-01
影响因子：
3.1
通讯作者：
LINDBERG, A
中科院分区：
文献类型：
--
作者：
FORSGREN, J;SAMUELSON, A;LINDBERG, A
DNA寡聚体5 '-d（TGCGGCCTCTCAGTCCCGCACTTTCATCTTCC）-3特异性识别流感嗜血杆菌16 S rRNA。我们在这里报告使用这种寡核苷酸，在其5'端标记荧光素标记，作为探针原位检测未封装的不可分型的H。10例临床上无感染但因鼻塞而切除腺样体的儿童的腺样体组织切片中检出流感病毒。在某些情况下，网状隐窝上皮局灶性浸润H。流感。在所有10例病例中，这些细菌定植的储库（很可能长期存在）似乎是在上皮下层中发现的巨噬细胞样细胞。这些单核细胞含有高达200个细胞内H。流感病毒细胞在透射电子显微镜下，可见巨噬细胞样细胞，胞内细菌呈球形形态，至少其中一些正在分裂。腺样细胞悬液，通过使用涂有抗CD 14标记物的单克隆抗体的顺磁珠富集巨噬细胞，产生高达1,100 CFU的不可分型H。在用庆大霉素杀死细胞外细菌，然后机械裂解细胞后，每10（5）个细胞中存在流感病毒。
The DNA oligomer 5'-d(TGCGGCCTCTCAGTCCCGCACTTTCATCTTCC)-3 specifically recognizes Haemophilus influenzae 16S rRNA. We report here the use of this oligonucleotide, with a fluorescein label tagged on its 5' end, as a probe for the in situ detection of nonencapsulated nontypeable H. influenzae in sections of adenoid tissue from 10 children who were clinically infection free but were having their adenoids removed because of nasal obstruction. In some cases, the reticular crypt epithelium was focally infiltrated by H. influenzae. The reservoir for these bacterial colonizations, in all likelihood long standing, seemed to be macrophage-like cells found in the subepithelial layers in all 10 cases. These mononuclear cells contained up to 200 intracellular H. influenzae cells. In the transmission electron microscope, macrophage-like cells with intracellular bacteria with coccoid morphology, at least some of which were dividing, were seen. Adenoid cell suspensions, enriched for macrophages by use of paramagnetic beads coated with monoclonal antibodies against the CD14 marker, yielded up to 1,100 CFU of nontypeable H. influenzae per 10(5) cells after killing of extracellular bacteria with gentamicin followed by mechanical lysis of the cells.