Rheological Properties, Drug Release Behavior and Cytocompatibility of Novel Hydrogels Prepared from Carboxymethyl Chitosan
Rheological Properties, Drug Release Behavior and Cytocompatibility of Novel Hydrogels Prepared from Carboxymethyl Chitosan
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DOI：
10.6023/a14100710
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发表时间：
2015-01
影响因子：
2.5
通讯作者：
Liu Shuilian;Zhou Yang;C. Fuhua;Zhu Shoujin;S. Feng;L. Suming
中科院分区：
文献类型：
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作者：
Liu Shuilian;Zhou Yang;C. Fuhua;Zhu Shoujin;S. Feng;L. Suming
以1-乙基-3-（3-二甲氨基丙基）-1-碳二亚胺/N-羟基琥珀酰亚胺（EDC/NHS）为催化剂，在温和条件下交联羧甲基壳聚糖（CMCS）制备水凝胶。所得水凝胶呈现不同的交联密度，这取决于EDC/NHS的量。流变学研究表明，高交联密度的水凝胶比低交联密度的水凝胶具有更高的储能模量。将模型药物胸腺五肽（TP-5）装载在CMCS水凝胶中。在所有情况下都获得抛物线释放曲线。高交联密度的水凝胶比低交联密度的水凝胶具有更致密的结构，因此具有更慢的释放速率。采用MTT法初步评价了CMCS水凝胶的细胞毒性。细胞形态学和RGR结果均表明，CMCS水凝胶具有非常低的毒性。因此，CMCS水凝胶在组织工程和药物控释领域具有广阔的应用前景。
Hydrogels were prepared by crosslinking carboxymethyl chitosan (CMCS) using 1-ethyl-3-(3-dimethylaminopropyl)-1-carbodiimide/N-hydroxysuccinimide (EDC/NHS) as catalyst under mild conditions. The resulting hydrogels present different crosslinking density, depending on the amount of EDC/NHS. Rheological studies show that hydrogels with high crosslinking density present higher storage modulus than those with low crosslinking density. A model drug, thymopentin (TP-5) is loaded in CMCS hydrogels. Parabolic release profiles are obtained in all cases. High crosslinking density hydrogels present slower release rate as compared to low crosslinking density ones because the former exhibits more compact structure. Preliminary studies were performed to evaluate the cytotoxicity of CMCS hydrogels by using MTT assay. Both cell morphology and RGR results show that the CMCS hydrogels present very low toxicity. Therefore, CMCS hydrogels are promising for applications in the fields of tissue engineering and controlled drug delivery.