Nuclear actin and myosins in adenovirus infection.
Nuclear actin and myosins in adenovirus infection.
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DOI：
10.1016/j.yexcr.2015.07.025
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发表时间：
2015-11-01
影响因子：
3.7
通讯作者：
de Lanerolle P
中科院分区：
文献类型：
--
作者：
Fuchsova B;Serebryannyy LA;de Lanerolle P
腺病毒血清型已被证明在感染过程中会导致核组织（包括转录机制）发生巨大变化。腺病毒破坏宿主细胞转录的能力有利于病毒复制。由于核肌动蛋白和核肌球蛋白 I、肌球蛋白 V 和肌球蛋白 VI 被认为是转录的直接调节因子和其他病毒复制的重要因子，因此我们试图确定核肌动蛋白和肌球蛋白如何参与腺病毒感染。我们首先确认了宿主转录机制重组为病毒复制中心。我们发现核肌动蛋白也会在病毒感染的中间阶段重组到转录位点，但不会在病毒感染的晚期阶段重组。此外，核肌球蛋白 I 与核肌动蛋白和病毒复制中心的转录位点一起定位。有趣的是，核肌球蛋白 V 和 VI 也重组为病毒复制中心，表现出不同的定位模式，表明这些核肌球蛋白具有特殊的作用。最后，我们评估了肌动蛋白在腺病毒感染中的作用，发现细胞质和核肌动蛋白可能在腺病毒感染和复制中发挥作用。我们的数据共同表明肌动蛋白和多种肌球蛋白参与腺病毒的核复制和晚期病毒基因表达。
Adenovirus serotypes have been shown to cause drastic changes in nuclear organization, including the transcription machinery, during infection. This ability of adenovirus to subvert transcription in the host cell facilitates viral replication. Because nuclear actin and nuclear myosin I, myosin V and myosin VI have been implicated as direct regulators of transcription and important factors in the replication of other viruses, we sought to determine how nuclear actin and myosins are involved in adenovirus infection. We first confirmed reorganization of the host’s transcription machinery to viral replication centers. We found that nuclear actin also reorganizes to sites of transcription through the intermediate but not the advanced late phase of viral infection. Furthermore, nuclear myosin I localized with nuclear actin and sites of transcription in viral replication centers. Intriguingly, nuclear myosins V and VI, which also reorganized to viral replication centers, exhibited different localization patterns, suggesting specialized roles for these nuclear myosins. Finally, we assessed the role of actin in adenovirus infection and found both cytoplasmic and nuclear actin likely play roles in adenovirus infection and replication. Together our data suggest the involvement of actin and multiple myosins in the nuclear replication and late viral gene expression of adenovirus.