Exploring the metabolic and genetic control of gene expression on a genomic scale
Exploring the metabolic and genetic control of gene expression on a genomic scale
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DOI：
10.1126/science.278.5338.680
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发表时间：
1997-10-24
期刊：
影响因子：
56.9
通讯作者：
Brown, PO
中科院分区：
文献类型：
--
作者：
DeRisi, JL;Iyer, VR;Brown, PO
利用包含酿酒酵母几乎所有基因的DNA微阵列，对从发酵到呼吸代谢转变过程中基因表达的时间程序进行了全面的研究。已知的具有代谢功能的基因的表达谱指向了在二氧基转移过程中发生的代谢重编程的特征，许多以前未被描述的基因的表达模式为它们可能的功能提供了线索。同样的DNA微阵列也被用来识别其表达受到转录共抑制因子Tup1缺失或转录激活因子YAP1过表达影响的基因。这些结果证明了这种方法在全基因组范围内探索基因表达模式的可行性和实用性。
DNA microarrays containing virtually every gene of Saccharomyces cerevisiae were used to carry out a comprehensive investigation of the temporal program of gene expression accompanying the metabolic shift from fermentation to respiration. The expression profiles observed for genes with known metabolic functions pointed to features of the metabolic reprogramming that occur during the diauxic shift, and the expression patterns of many previously uncharacterized genes provided clues to their possible functions. The same DNA microarrays were also used to identify genes whose expression was affected by deletion of the transcriptional co-repressor TUP1 or overexpression of the transcriptional activator YAP1. These results demonstrate the feasibility and utility of this approach to genomewide exploration of gene expression patterns.