Effect of multiple irradiation with low doses of gamma-rays on morphological transformation and growth ability of human embryo cells in vitro.
Effect of multiple irradiation with low doses of gamma-rays on morphological transformation and growth ability of human embryo cells in vitro.
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低剂量伽玛射线多次照射对体外人胚胎细胞形态转化及生长能力的影响
DOI：
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发表时间：
1992
影响因子：
2.6
通讯作者：
K. Watanabe
中科院分区：
文献类型：
--
作者：
M. Watanabe;Misa Suzuki;Keiji Suzuki;K. Nakano;K. Watanabe
我们已经测量了在人胚胎（HE）细胞中的转化表型的表达，这些细胞在体外的整个生命周期中每周重复照射7.5 cGy的剂量。重复照射直至细胞累积到195 cGy，此时细胞已达到其第26代的等效值，并通过集落形成测定几代细胞样品的细胞存活、次黄嘌呤鸟嘌呤磷酸核糖转移酶（HGPRT）基因座的突变和通过病灶形成测定转化。总剂量为97.5 cGy的多次辐照培养物的寿命（平均群体倍增）略高于对照组，但显著延长。例如，如果细胞累积了195 cGy，则体外HE细胞的最大细胞分裂数扩展到未照射对照的130-160%。虽然在细胞累积97.5 cGy之前未观察到细胞转化灶，但随着累积剂量的增加而增加。然而，没有细胞在体外表现出无限的寿命，也表达了致瘤性。
We have measured expression of transformed phenotypes in human embryo (HE) cells repeatedly irradiated with a dose of 7.5 cGy per week throughout the life span of these cells in vitro. Irradiation was repeated until the cells had accumulated 195 cGy at which time the cells had reached the equivalent of their 26th passage and samples of cells at several passages were assayed for cell survival by colony formation, for mutation at hypoxanthine guanine phosphoribosyl transferase (HGPRT) locus and for transformation by focus formation. The lifespan (mean population doublings) of multiple irradiated cultures with a total dose of 97.5 cGy was slightly, but significantly, prolonged over that of controls. For example, if cells had accumulated 195 cGy, the maximum number of cell division of HE cells in vitro extended to 130-160% of non-irradiated control. Although transformed foci were not observed with cells until cells had accumulated 97.5 cGy, it increased with increasing accumulated dose. No cells, however, showed unlimited life span in vitro and also expressed tumorigenicity.
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