Improvements in live cell analysis of G protein coupled receptors using second generation BD calcium assay kits.
Improvements in live cell analysis of G protein coupled receptors using second generation BD calcium assay kits.
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DOI：
10.2174/1875397300802010010
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发表时间：
2008-10-10
期刊：
影响因子：
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通讯作者：
Brasch M
中科院分区：
文献类型：
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作者：
Li X;Llorente I;Brasch M
BD™钙测定试剂盒专为基于细胞的钙动员高通量筛选分析而设计。试剂盒使用专利配方，包括一个非荧光钙指示剂，该指示剂在细胞内激活，并在钙结合时显示增强的荧光。该配方包括一种信号增强剂，以BD开发的一项技术为基础，以均质、免清洗的分析格式最大化背景上的信号。我们已经将下一代BD钙分析试剂盒产品系列与该配方的前几个版本以及其他商业上可用的均质钙分析试剂盒进行了比较。这些改进使我们在所有平板格式(包括1536)中测试的细胞系和受体具有更好的性能。
BD™ Calcium Assay Kits are designed for cell-based calcium mobilization high-throughput screening assays. The kits use a proprietary formulation including a non-fluorescent calcium indicator that becomes activated inside the cell and shows increased fluorescence upon calcium binding. The formulation includes a signal-enhancing reagent to maximize the signal over background in a homogeneous, no-wash assay format, based on a technology developed at BD. We have compared the next generation BD calcium assay kit product family to previous versions of the formulation, and to other commercially available homogeneous calcium assay kits. The improvements have enabled better performance on the cell lines and receptors that we have tested in all plate formats including 1536.