HUMAN SKELETAL-MUSCLE - PROPERTIES OF CHEMICALLY SKINNED FIBER
HUMAN SKELETAL-MUSCLE - PROPERTIES OF CHEMICALLY SKINNED FIBER
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DOI：
10.1126/science.187.4181.1075
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发表时间：
1975-01-01
期刊：
影响因子：
56.9
通讯作者：
BRANDT, PW
中科院分区：
文献类型：
--
作者：
WOOD, DS;ZOLLMAN, J;BRANDT, PW
描述了用于不可逆地破坏人骨骼肌的肌纤维膜并允许钙和其它可扩散溶质（如三磷酸腺苷）进入肌丝空间的“剥皮”程序。当暴露于离子钙时，即使在5°C下储存1至2周后，单皮层纤维也会产生约1.5千克/平方厘米的等距张力。长达5天的准备将螯合，并在适当的条件下（阴离子替代，咖啡因添加，或镁撤出），释放钙。细胞内钙分布和钙诱导的激活的收缩蛋白的调节进行了讨论，并与人类纤维的形态和其他肌肉制剂中发生的类似过程。
A "skinning" procedure is described for irreversibly disrupting the sarcolemmal membrane of human skeletal muscle and allowing calcium and other diffusible solutes (such as adenosine triphosphate) access to the myofilament space. Single skinned fibers give isometric tensions of about 1.5 kilograms per square centimeter when exposed to ionized calcium even after 1 to 2 weeks of storage at 5°C. For up to 5 days the preparation will sequester and, under appropriate conditions (anion substitution, caffeine addition, or magnesium withdrawal), release calcium. The regulation of intracellular calcium distribution and the calcium-induced activation of the contractile proteins are discussed and related to the morphology of human fibers and to similar processes occurring in other muscle preparations.