Antibody-dependent cellular cytotoxicity to target cells infected with herpes simplex viruses: functional adequacy in the neonate.
Antibody-dependent cellular cytotoxicity to target cells infected with herpes simplex viruses: functional adequacy in the neonate.
复制标题
对感染单纯疱疹病毒的靶细胞的抗体依赖性细胞毒性：新生儿的功能充分性。
作者：
S. L. Shore;H. Milgrom;P. Wood;A. Nahmias
用51Cr释放试验评估了人类新生儿抗体依赖性细胞毒性（ADCC）的功能充分性，该试验以急性感染1型或2型单纯疱疹病毒（HSV）的组织培养细胞为靶标。评估了ADCC的两个方面：脐带血单个核细胞（MC）的细胞毒性效应活性和介导ADCC通过胎盘的抗体能力。虽然在所有13个测试的脐带血样本中很容易检测到效应细胞活性，但在相同的效应细胞：靶细胞比例下，脐带血MC与正常成人的血液MC相比，显示出适度降低的细胞毒性活性。这一发现表明，脐带血中的效应细胞占循环MC总数的比例降低，可能与新生儿对HSV感染的易感性增加有关。另一方面，脐带血中MC的数量几乎是成人血液的两倍，这表明脐带血中ADCC效应细胞的绝对数量在成人范围内。介导ADCC对抗hsv感染细胞的抗体被证明可以定量地通过胎盘转移，进一步证明它是一种IgG免疫球蛋白。
The functional adequacy of antibody-dependent cellular cytotoxicity (ADCC) in the human neonate was evaluated in a 51Cr release assay which employs tissue culture cells acutely infected with type 1 or type 2 herpes simplex virus (HSV) as targets. Two aspects of ADCC were assessed: cytotoxic effector activity in cord blood mononuclear cells (MC) and the ability of the antibody mediating ADCC to pass the placenta. Although effector cell activity was readily detected in all 13 cord blood specimens tested, cord blood MC showed moderately reduced cytotoxic activity when compared with blood MC from normal adults at the same effector cell:target cell ratio. This finding suggests that effector cells in cord blood make up a reduced proportion of the total circulating MC population and may be of relevance to the newborn's increased susceptibility to HSV infection. On the other hand, the number of MC in cord blood was found to be almost twice that of adult blood, suggesting that the absolute number of ADCC effector cells in cord blood was within the adult range. The antibody mediating ADCC to HSV-infected cells was shown to be transferred quantitatively across the placenta, providing further evidence that it is an IgG immunoglobulin.