Youthful systemic milieu alleviates renal ischemia-reperfusion injury in elderly mice
Youthful systemic milieu alleviates renal ischemia-reperfusion injury in elderly mice
复制标题
年轻的全身环境减轻老年小鼠的肾缺血再灌注损伤
DOI：
10.1016/j.kint.2018.03.019
复制
发表时间：
2018-08-01
影响因子：
19.6
通讯作者：
Chen, Xiangmei
中科院分区：
文献类型：
--
作者：
Liu, Dong;Lun, Lide;Chen, Xiangmei
急性肾损伤（阿基）是老年人发病率高、防治困难的疾病，其主要原因之一是肾缺血再灌注损伤（IRI）。一个年轻的系统环境可以防止衰老的器官。然而，尚不清楚年轻环境是否可以减少老年人的肾脏IRI。为了研究这个问题，在建立联体共生模型后三周，在老年（24个月）小鼠中诱导双侧肾IRI。在IRI后24小时，与老年野生型小鼠相比，患有IRI的老年小鼠具有显著的肾组织学损伤、肾功能下降、氧化应激增加、炎症和细胞凋亡。然而，自噬没有增加。与患有IRI的老年小鼠相比，患有IRI的老年-老年联体共生小鼠在肾组织学损伤、氧化应激、炎症、细胞凋亡或自噬方面没有表现出差异，但确实表现出肾功能改善。与高龄联体小鼠IRI相比，青年联体小鼠（12周龄）IRI的老年小鼠肾组织损伤轻，肾功能好。肾脏氧化应激、炎症和细胞凋亡显著减少，自噬显著增加。因此，年轻的全身环境可能会减少氧化应激，炎症和细胞凋亡，并增加自噬老年小鼠与IRI。这些作用改善了老年小鼠的IRI损伤。我们的研究为有效预防和治疗老年阿基提供了新的思路。
The incidence of acute kidney injury (AKI) is high in elderly people, and is difficult to prevent and treat. One of its major causes is renal ischemia-reperfusion injury (IRI). A young systemic environment may prevent the senescence of old organs. However, it is unknown whether a young milieu may reduce renal IRI in the elderly. To examine this question, bilateral renal IRI was induced in old (24 months) mice three weeks after parabiosis model establishment. At 24 hours after IRI, compared to old wild-type mice, the old mice with IRI had significantly damaged renal histology, decreased renal function, increased oxidative stress, inflammation, and apoptosis. However, there was no increase in autophagy. Compared to old mice with IRI, old-old parabiosis mice with IRI did not show differences in renal histological damage, oxidative stress, inflammation, apoptosis, or autophagy, but did exhibit improved renal function. Compared to the old-old parabiosis mice with IRI, the old mice with IRI in the young (12 week)-old parabiosis showed less renal histological injury and better renal function. Renal oxidative stress, inflammation, and apoptosis were significantly decreased, and autophagy was significantly increased. Thus, a youthful systemic milieu may decrease oxidative stress, inflammation, and apoptosis, and increase autophagy in old mice with IRI. These effects ameliorated IRI injuries in old mice. Our study provides new ideas for effectively preventing and treating AKI in the elderly.