Low Molecular Weight Polyethylenimine Conjugated Gold Nanoparticles as Efficient Gene Vectors
Low Molecular Weight Polyethylenimine Conjugated Gold Nanoparticles as Efficient Gene Vectors
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低分子量聚乙烯亚胺共轭金纳米粒子作为高效基因载体
DOI：
10.1021/bc900374d
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发表时间：
2010-05-01
影响因子：
4.7
通讯作者：
Zhuo, Renxi
中科院分区：
文献类型：
--
作者：
Hu, Chu;Peng, Qi;Zhuo, Renxi
合成了与低分子量聚乙烯亚胺（PEI 800 Da）缀合的金纳米颗粒（GNP），并研究了它们作为基因转染载体的特性。聚乙烯亚胺偶联的 GNP (GNP-PEI800s) 可以在琼脂糖凝胶电泳中 N/P 比大于 4 时完全延迟质粒 DNA，并且还可以有效保护 DNA 免受 DNase 的攻击。 TEM 成像显示，与较低接枝密度的 GNP-PEI800 相比，具有较高 PEI 接枝密度的 GNP-PEI800 与质粒 DNA 形成的复合物更紧凑、更小。这些复合物在体外猴肾细胞中显示出高效率的基因传递。在没有血清的情况下，GNP-PEI800s 可以将 pGL-3 转染至 COS-7 细胞，效率比未修饰的 PEI800 高 3 至 4 个数量级，几乎达到与 PEI 25 kDa 相同的量级。更重要的是，与高分子量 PEI（例如 PEI 25 kDa）在 scrum 存在下效率显着降低相比，GNP-PEI800s 的效率在含血清培养基中可以保留甚至增强。 GNP-PEI800 1.3 在 10% 血清培养基中的转染效率是 PEI 25 kDa 的 60 倍以上。与 PEI 25 kDa 相比，所有 GNP-PEI800 均表现出轻微的细胞毒性。
Gold nanoparticles (GNPs) conjugated with low molecular weight polyethylenimine (PEI 800 Da) were synthesized, and their characteristics as gene transfection vectors were investigated. The polyethylenimine conjugated GNPs (GNP-PEI800s) can retard plasmid DNA completely at N/P ratios above 4 in electrophoresis on agarose gel, and they also render effective protection of DNA from attack by DNase. TEM imaging revealed that GNP-PEI800s with higher PEI grafting density resulted in more compact and smaller complexes with plasmid DNA, compared to those obtained with lower grafting density ones. These complexes showed high efficiency in gene delivery in monkey kidney cells in vitro. In the absence of serum, GNP-PEI800s can transfect pGL-3 to COS-7 cells 3 to 4 orders more efficient than unmodified PEI800, reaching almost the same magnitude of PEI 25 kDa. More importantly, in contrast to the dramatically lowered efficiency of high molecular weight PEIs such as PEI 25 kDa in the presence of scrum, the efficiency of GNP-PEI800s can be retained or even enhanced in serum-containing media. GNP-PEI800 1.3 exhibited transfection efficiency exceeding 60-fold that of PEI 25 kDa in 10% serum medium. All GNP-PEI800s exhibit mild cytotoxicity in comparison with that of PEI 25 kDa.