One-Pot Dual-Labeling of a Protein by Two Chemoselective Reactions
One-Pot Dual-Labeling of a Protein by Two Chemoselective Reactions
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DOI：
10.1002/anie.201100840
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发表时间：
2011-01-01
影响因子：
16.6
通讯作者：
Wu, Yao-Wen
中科院分区：
文献类型：
--
作者：
Yi, Long;Sun, Hongyan;Wu, Yao-Wen
蛋白质的双系统：通过化学选择性天然化学连接和肟连接的组合，在一锅法中实现了Rab GT3的位点特异性双色标记。这种策略可能是一种通用的，简便的，有效的方法，特定的多个修饰的给定蛋白质。Rab GT生物传感器被证明用于蛋白质折叠和蛋白质-蛋白质相互作用研究。© 2011 Wiley-VCH Verlag GmbH & Co. KGaA，魏因海姆。
Dual system for proteins: The site-specific two-color labeling of a Rab GTPase was achieved in a one-pot procedure by combination of chemoselective native chemical ligation and oxime ligation. This strategy could be a general, facile, and efficient method for specific multiple modifications of a given protein. The Rab GTPase biosensor was demonstrated for protein folding and protein-protein interaction studies.© 2011 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.