Ionic topochemical tuned biosensor interface.
Ionic topochemical tuned biosensor interface.
复制标题
离子拓扑化学调谐生物传感器接口。
DOI：
10.1021/la9047215
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
M. Katterle
中科院分区：
文献类型：
--
作者：
B. Nagel;N. Gajovic;F. Scheller;M. Katterle
合成了两种新的亲水性聚乙二醇（PEG）基氧化还原共聚物，其具有电化学活性的二茂铁（Fc）和硫醇/二硫键锚定功能。这些共聚物被证明吸附在金表面上，导致聚合物自组装单层（pSAMs），具有三重功能：“氧化还原活性”，“离子可调”，和“生物惰性”。两种固定化聚合物的氧化还原电位均为+400 mV（Ag| AgCl），促进NADH的电催化氧化。此外，聚合物的界面结构受到Ca（2+）浓度增加的影响，这导致电化学响应的放大。在加入10 mM Ca（2+）离子后，测量NADH氧化的电极电流增加了80%。考虑到Ca（2+）对非均相电子转移的影响，基于非环状PEG部分的强螯合能力，提出了固定化聚合物的结构模型。
Two new hydrophilic, poly(ethylene glycol) (PEG)-based redox copolymers bearing electrochemically active ferrocene (Fc) and thiol/disulfide anchoring functionalities were synthesized. These copolymers are shown to adsorb on gold surfaces causing polymeric self-assembled monolayers (pSAMs) that possess triple functions: "redox-active", "ionic-tunable", and "bio-inert". Both immobilized polymers showed redox potentials at +400 mV (Ag|AgCl), and facilitate the electrocatalytical oxidation of NADH. Additionally, interfacial architecture of the polymers is affected by an increase in Ca(2+) concentration, which leads to an amplification of the electrochemical response. The electrode current, measured for NADH-oxidation, increased by 80% after addition of 10 mM Ca(2+) ions. Considering the Ca(2+) influence on the heterogeneous electron transfer a structural model of the immobilized polymers is proposed based on the strong chelating ability of noncyclic PEG moieties.