Effects of p,p′-DDE and some other chlorinated hydrocarbons on the formation of prostaglandins by the avian eggshell gland mucosa
Effects of p,p′-DDE and some other chlorinated hydrocarbons on the formation of prostaglandins by the avian eggshell gland mucosa
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p,p-DDE 和其他一些氯化烃对禽蛋壳腺粘膜形成前列腺素的影响
DOI：
10.1007/bf02035127
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发表时间：
2005
影响因子：
6.1
通讯作者：
M. Bartonek
中科院分区：
文献类型：
--
作者：
C. Lundholm;M. Bartonek
本文研究了几种结构上相关的氯代烃类化合物对鸭和家禽蛋壳腺粘膜产生三尖杉酯碱的影响。体外实验表明，p，p′-DDE、Arochlor 1242和Arochlor 1260均能显著抑制家禽蛋壳腺粘膜匀浆PGF_（2 α）、PGE_（2 β）和TxB_（2 β）的生成，但p，p′-DDT和o，p′-DDE对PGF_（2 α）、PGE_（2 β）和TxB_（2 β）的生成无明显影响。相比之下，在鸭蛋壳腺粘膜匀浆中，相同的洋地黄素的合成对体外加入的5 M M p，p′-DDE的抑制更为敏感。卵壳腺粘膜合成PGF_（2 α）、PGE_（2 β）和TxB_（2 β）的量显著高于大胃区和峡部粘膜。鸭蛋壳腺粘膜组织匀浆中前列腺素的合成量显著高于家禽组织匀浆中前列腺素的合成量，且08：00屠宰时PGF 2 α的合成量显著高于16：00屠宰时。在鸭中，与同期对照值相比，40 ppm，p，p′-DDE的饲料给药45天导致蛋壳变薄21%。与对照组相比，该处理还导致蛋壳腺粘膜匀浆中的显著影响：Ca 2+摄取减少43%，PGF 2 α、PGE 2和TxB 2的合成分别减少26%、38%和53%，Ca含量增加至145%。p，p′-DDE在抑制蛋壳腺中前列腺素形成中的作用被讨论为这种氯代烃的蛋壳变薄作用的机制。
Some structurally related chlorinated hydrocarbons were investigated for their effects on the production of prostaglandins by the eggshell gland mucosa of ducks and domestic fowl. Formation of PGF2α, PGE2 and TxB2 by homogenates of domestic fowl eggshell gland mucosa was significantly inhibited by in vitro addition of p,p′-DDE, Arochlor 1242 and, to a lesser extent, Arochlor 1260, but not by p,p′-DDT and o,p′-DDE. Comparatively, in duck eggshell gland mucosa homogenates, synthesis of the same prostaglandins was somewhat more sensitive to inhibition by 5 ΜM p,p′-DDE added in vitro. Eggshell gland mucosa synthesized significantly more PGF2α, PGE2 and TxB2 than did the mucosa of the magnum and isthmus regions of the oviduct. Duck eggshell gland mucosa homogenates synthesized significantly more prostaglandins than similar homogenates from the domestic fowl, and, considering the former synthesis of PGF2α was significantly higher when ducks were slaughtered at 08:00 than at 16:00 hours. In ducks, dietary administration of 40 ppm, p,p′-DDE for 45 days resulted in 21% eggshell thinning compared to the contemporary control values. This treatment also resulted in notable effects in homogenates of the eggshell gland mucosa, as compared to controls: Ca2+ uptake was reduced by 43%, synthesis of PGF2α, PGE2 and TxB2 was reduced by 26%, 38% and 53%, respectively; the Ca content was increased to 145%. The role of p,p′-DDE in inhibiting prostaglandin formation in the eggshell gland is discussed as a mechanism of the eggshell thinning action of this chlorinated hydrocarbon.