Ectopic expression of VyCIPK1 gene, isolated from wild grape Vitis yanshanesis J, X. Chen., confers the tolerance to salt in transgenic tobacco
Ectopic expression of VyCIPK1 gene, isolated from wild grape Vitis yanshanesis J, X. Chen., confers the tolerance to salt in transgenic tobacco
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从野生葡萄 Vitis yanshanesis J, X. Chen. 中分离出的 VyCIPK1 基因的异位表达赋予转基因烟草耐盐性
DOI：
10.1080/17429145.2022.2115158
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发表时间：
2022-08
影响因子：
3.2
通讯作者：
Bo Li
中科院分区：
文献类型：
--
作者：
Ziguo Zhu;Lingmin Dai;Guangxia Chen;Guanghui Yu;Xiujie Li;Zhen Han;Bo Li
钙调素样相互作用蛋白激酶在植物对非生物胁迫的响应和发育中起重要作用。但CIPK基因在葡萄藤中的作用尚不清楚。本研究从中国野生葡萄V. Yanshanesis中分离得到的VyCIPK1受盐胁迫强烈诱导。过表达VyCIPK1可诱导AOC和AOS，并导致烟草中jamonate水平显著升高。在盐胁迫下，转基因植株的发芽率、叶片数和鲜重均高于野生型植株。在盐胁迫条件下，转基因植株的叶绿素含量、过氧化氢酶活性、过氧化物酶活性、超氧化物歧化酶活性均高于野生型，丙二醛含量、H2O2和O2-含量均低于野生型。胁迫相关基因ERD10C、ERD10D、LEA5、POD、SOD和CAT在转基因植株中表达上调。我们的研究结果表明，VyCIPK1具有作为葡萄耐盐分子育种候选基因的潜力。
ABSTRACT Calmodulin-like interacting protein kinases play an important role in plant response to abiotic stresses and development. But the role of the CIPK gene in grapevine is unknown. In this study, VyCIPK1, isolated from the Chinese wild grape V. Yanshanesis, was strongly induced by salt stress. Overexpressing VyCIPK1 could induce AOC and AOS, and result in notably increased jamonate levels in tobacco. Under salt stress, transgenic plants showed higher germination rate, leaf number, and fresh weight than wild-type plants. Moreover, transgenic plants displayed higher chlorophyll content, catalase activity, peroxidase activity, superoxide dismutase activity, and lower malondialdehyde content, H2O2, and O2- content than that of wild type under salt stress conditions. And the stress-related genes, including ERD10C, ERD10D, LEA5, POD, SOD, and CAT, were up-regulated in transgenic plants. Our founding demonstrated that the VyCIPK1 has the potential for grape molecular breeding of salt tolerance as a candidate gene.
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