Fidelity of DNA replication catalysed in vitro on a natural DNA template by the T4 bacteriophage multi-enzyme complex
Fidelity of DNA replication catalysed in vitro on a natural DNA template by the T4 bacteriophage multi-enzyme complex
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T4 噬菌体多酶复合物在天然 DNA 模板上体外催化 DNA 复制的保真度
作者：
U. Hibner;B. Alberts
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More than 50 copies of a ΦX174 DNA template can be made in 60 min in an in vitro DNA replication system consisting of seven purified replication proteins isolated from T4 bacteriophage-infected cells. By transfecting with the DNA products and assaying for the reversion of specific amber mutants, the high degree of base-pairing fidelity in this system is revealed; the in vitro system is also shown to respond to the mutagenic effect of Mn2+ and to display strong base-pair context effects on fidelity, as expected from in vivo studies.