Identification of a pituitary factor responsible for enhancement of plasminogen activator activity in breast tumor cells.
Identification of a pituitary factor responsible for enhancement of plasminogen activator activity in breast tumor cells.
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鉴定负责增强乳腺肿瘤细胞中纤溶酶原激活剂活性的垂体因子。
DOI：
10.1073/pnas.83.20.7780
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发表时间：
1986
影响因子：
11.1
通讯作者：
Ossowski,L
中科院分区：
文献类型：
--
作者：
Mira-y-Lopez,R;Joseph-Silverstein,J;Rifkin,DB;Ossowski,L
绵羊脑垂体含有一种蛋白质，在T47D、MTW9/PL和SC115乳腺肿瘤细胞的无血清培养物中可刺激纤溶酶原激活剂（PA）活性3至20倍。发现这种蛋白质在大小、阳离子性质和对肝素的亲和力方面与碱性成纤维细胞生长因子（bFGF）相似。纯化的人胎盘bFGF，人和牛垂体bFGF的同源物，在1 ng/ml的浓度下有效刺激乳腺肿瘤细胞PA。胎盘bFGF的抗体阻断了绵羊垂体提取物的PA刺激活性。由于这些特性，绵羊垂体中的活性蛋白被鉴定为bFGF。这代表了bFGF的另一个功能，表明其靶细胞谱比以前认为的更宽。由于先前建立的PA生产在体外和肿瘤生长在体内之间的相关性，我们认为，bFGF可能有助于乳腺癌在体内的生长。其他类型的癌症也可能受到bFGF的影响。
Sheep pituitary glands contain a protein that stimulates plasminogen activator (PA) activity 3- to 20-fold in serum-free cultures of T47D, MTW9/PL, and SC115 breast tumor cells. This protein was found to be similar to basic fibroblast growth factor (bFGF) in size, cationic nature, and affinity for heparin. Purified human placental bFGF, a homologue of human and bovine pituitary bFGF, was effective in stimulating mammary tumor cell PA at a concentration of 1 ng/ml. Antibodies to placental bFGF blocked the PA stimulatory activity of sheep pituitary extracts. Because of these properties, the active protein in sheep pituitary glands was identified as bFGF. This represents another function of bFGF, indicating that its spectrum of target cells is wider than previously thought. Because of previously established correlations between PA production in vitro and tumor growth in vivo, we suggest that bFGF may contribute to the growth of breast carcinomas in vivo. Other types of carcinomas may also be affected by bFGF.