Effects of platinum-thymine on mitosis of sarcoma-180 ascites cells in vitro: ultrastructural study.
Effects of platinum-thymine on mitosis of sarcoma-180 ascites cells in vitro: ultrastructural study.
复制标题
铂-胸腺嘧啶对肉瘤 180 腹水细胞体外有丝分裂的影响：超微结构研究。
作者：
Ofosu,G;Bowden,R
用浓度为 60 μg/ml 的铂-胸腺嘧啶处理后的 sarcoma-180 腹水细胞的电子显微镜研究显示有丝分裂受到抑制。该药物将染色体聚集在细胞中心，形成致密的团块，防止它们在有丝分裂过程中分离。长时间的处理使纺锤纤维和细胞质微丝解聚。退化的细胞质细胞器聚集在聚集的染色体周围。皮质细胞质的崩解和脱落是明显的。铂胸腺嘧啶处理的细胞无论在新鲜培养基中保留多长时间都不会恢复有丝分裂。铂胸腺嘧啶对有丝分裂机制的干扰抑制了有丝分裂过程并导致癌细胞最终死亡。
Electron microscopic studies of sarcoma-180 ascites cells, which were treated with platinum-thymine at a concentration of 60 μg/ml, showed mitotic inhibition. The drug clumped the chromosomes into a compact mass at the center of the cell preventing them from separating during mitosis. Prolonged treatment depolymerized spindle fibers and cytoplasmic microfilament. Degraded cytoplasmic organelles crowded around the clumped chromosomes. Disintegrating and casting off of cortical cytoplasm was apparent. Platinum-thymine-treated cells did not resume mitosis irrespective of the amount of time they were allowed to remain in fresh medium. Perturbation of mitotic machinery by platinum-thymine inhibited the mitotic process and caused eventual demise of the cancer cells.