Interactions of IgG from SLE patients with peripheral blood mononuclear cells and adherent cell populations.
Interactions of IgG from SLE patients with peripheral blood mononuclear cells and adherent cell populations.
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SLE 患者的 IgG 与外周血单核细胞和贴壁细胞群的相互作用。
DOI：
10.1007/bf00541345
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发表时间：
1988
影响因子：
4
通讯作者：
WilliamsJr,RC
中科院分区：
文献类型：
--
作者：
Ramirez,F;Searles,RB;WilliamsJr,RC
研究了来自正常对照受试者的外周血单个核细胞（PBMC）与从正常或活动性系统性红斑狼疮（SLE）血清中分离的IgG的相互作用。用0.5-1.0 mg SLE IgG预孵育PBMC 24 h，然后洗涤，随后细胞培养7天，导致细胞上清液IgG的显著相对增加。在培养物中加入或不加入环孢菌素A或吲哚美辛的情况下观察到这些发现。使用分离的正常贴壁细胞群体的实验表明，SLE IgG，但不是正常的IgG，当与贴壁细胞巨噬细胞/单核细胞群体预孵育时，能够诱导后者产生抑制因子，能够下调IgG的产生或从与美洲商陆有丝分裂原培养的正常非贴壁细胞释放。这些研究结果表明，活动性SLE患者的IgG可能与IgG产生的PBMC群体以及粘附细胞群体相互作用，以影响随后培养的细胞的IgG产生或释放。
Peripheral blood mononuclear cells (PBMCs) from normal control subjects were studied for their interactions with IgG isolated from normal or active systemic lupus erythematosus (SLE) sera. Preincubation of PBMCs with SLE IgG at 0.5–1.0 mg for 24 h followed by washing and subsequent cell culture for 7 days resulted in marked relative increase in cell supernatant IgG. These findings were noted with and without inclusion of cyclosporin A or indomethacin in cultures. Experiments using isolated normal adherent cell populations showed that SLE IgG but not normal IgG, when preincubated with adherent cell macrophage/monocyte populations, was capable of inducing the latter to produce suppressor factors capable of down modulating IgG production or release from normal non-adherent cells cultured with pokeweed mitogen. These findings suggest that IgG from active SLE patients may interact with both IgG-producing PBMC populations as well as adherent-cell populations to influence IgG production or release from subsequently cultured cells.