Induced accumulation of 2-hydroxy-4,7-dimethoxy-1,4-benzoxazin-3-one glucoside (HDMBOA-Glc) in maize leaves
Induced accumulation of 2-hydroxy-4,7-dimethoxy-1,4-benzoxazin-3-one glucoside (HDMBOA-Glc) in maize leaves
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DOI：
10.1016/s0031-9422(00)00494-5
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发表时间：
2001-04-01
期刊：
影响因子：
3.8
通讯作者：
Iwamura, H
中科院分区：
文献类型：
--
作者：
Oikawa, A;Ishihara, A;Iwamura, H
用CuCl_2、壳戊糖、五-N-乙酰壳戊糖或茉莉酸（JA）处理玉米叶片，诱导2-（2-羟基-4，7-二甲氧基-1，4-苯并恶嗪-3-酮）-β-D-吡喃葡萄糖（HDM BOA-Glc）的积累。HDMBOA-Glc的蓄积伴随着2-（2，4-二羟基-7-甲氧基-1，4-苯并恶嗪-3-酮）-β-D-吡喃葡萄糖（DIMBOA-Glc）水平的降低。当在[Me-H-2（3）] L-甲硫氨酸存在下用JA处理叶片段时，标记物被有效地掺入HDMBOA-Glc中，而没有检测到掺入DIMBOA-Glc或HMBOA-Glc中，表明组成型DIMBOA-Glc通过在4位的甲基化转化为HDMBOA-Glc。内源性5.4及其亮氨酸缀合物的水平在用CuCl 2和壳五糖处理的叶段中积累HDMBOA-葡萄糖之前瞬时增加。脂氧合酶抑制剂布洛芬抑制了CuCl 2诱导的HDMBOA-葡萄糖积累，JA可恢复减少的HDMBOA-葡萄糖积累。这些发现表明JA在诱导HDMBOA-Glc积累中起信号转导器的作用。(C)2001爱思唯尔科技有限公司版权所有。
Accumulation of 2-(2-hydroxy-4,7-dimethoxy-1,4-benzoxazin-3-one)-beta -D-glucopyranose (HDM BOA-Glc) was induced in maize leaves by treatment with CuCl2, chitopentaose, penta-N-acetylchitopentaose, or jasmonic acid (JA). The accumulation of HDMBOA-Glc was accompanied by a decrease in level of 2-(2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one)-beta -D-glucopyranose (DIMBOA-Glc). When the leaf segments were treated with JA in the presence of [Me-H-2(3)]L-methionine, the label was efficiently incorporated into HDMBOA-Glc, while no incorporation into DIMBOA-Glc or HMBOA-Glc was detected, suggesting the conversion of constitutive DIMBOA-Glc to HDMBOA-Glc by methylation at the 4-position. Levels of endogenous 5.4 and its leucine conjugate transiently increased prior to the accumulation of HDMBOA-Glc in leaf segments treated with CuCl2 and chitopentaose. The lipoxygenase inhibitor ibuprofen suppressed the accumulation of HDMBOA-Glc induced by CuCl2 treatment, and the reduced accumulation of HDMBOA-Glc was recovered by addition of JA. These findings suggested that JA functions as a signal transducer in the induction of HDMBOA-Glc accumulation. (C) 2001 Elsevier Science Ltd. All rights reserved.