Clonal barcoding of endogenous adult hematopoietic stem cells reveals a spectrum of lineage contributions.
Clonal barcoding of endogenous adult hematopoietic stem cells reveals a spectrum of lineage contributions.
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DOI：
10.1073/pnas.2317929121
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发表时间：
2024-01-23
影响因子：
11.1
通讯作者：
Reizis, Boris
中科院分区：
文献类型：
--
作者：
Feng, Jue;Jang, Geunhyo;Esteva, Eduardo;Adams, Nicholas M.;Jin, Hua;Reizis, Boris
造血的分层模型假定自我更新的多能造血干细胞（HSC）产生所有血细胞谱系。虽然该模型解释了移植环境中的造血，但其对稳态造血的适用性仍有待澄清。在这里，我们使用内源性成体HSC的诱导型克隆DNA条形码来追踪它们对未操作动物中主要造血细胞谱系的贡献。虽然大多数条形码对于单一谱系是独特的，但我们也观察到多个谱系之间，特别是淋巴细胞和骨髓细胞之间频繁的条形码共享。这些结果表明，造血干细胞的单系和多系贡献共同驱动连续的造血，并强调了骨髓和淋巴发育的密切关系。
The hierarchical model of hematopoiesis posits that self-renewing, multipotent hematopoietic stem cells (HSCs) give rise to all blood cell lineages. While this model accounts for hematopoiesis in transplant settings, its applicability to steady-state hematopoiesis remains to be clarified. Here, we used inducible clonal DNA barcoding of endogenous adult HSCs to trace their contribution to major hematopoietic cell lineages in unmanipulated animals. While the majority of barcodes were unique to a single lineage, we also observed frequent barcode sharing between multiple lineages, specifically between lymphocytes and myeloid cells. These results suggest that both single-lineage and multilineage contributions by HSCs collectively drive continuous hematopoiesis, and highlight a close relationship of myeloid and lymphoid development.
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影响因子：
64.8
作者：
Rodriguez-Fraticelli AE;Wolock SL;Weinreb CS;Panero R;Patel SH;Jankovic M;Sun J;Calogero RA;Klein AM;Camargo FD
通讯作者：
Camargo FD
影响因子：
23.9
作者：
Dykstra, Brad;Kent, David;Eaves, Connie
通讯作者：
Eaves, Connie
影响因子：
64.5
作者：
Adolfsson, J;Månsson, R;Jacobsen, SEW
通讯作者：
Jacobsen, SEW
影响因子：
32.4
作者：
Sawai CM;Babovic S;Upadhaya S;Knapp DJHF;Lavin Y;Lau CM;Goloborodko A;Feng J;Fujisaki J;Ding L;Mirny LA;Merad M;Eaves CJ;Reizis B
通讯作者：
Reizis B
影响因子：
30.5
作者：
Chen, Edward L. Y.;Thompson, Patrycja K.;Zuniga-Pflucker, Juan Carlos
通讯作者：
Zuniga-Pflucker, Juan Carlos