Pharmacological characterization and region‐specific expression in brain of the β2‐ and β3‐subunits of the rat GABAA receptor
Pharmacological characterization and region‐specific expression in brain of the β2‐ and β3‐subunits of the rat GABAA receptor
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大鼠 GABAA 受体 β2 和 β3 亚基的药理学特征和脑内区域特异性表达
DOI：
10.1016/0014-5793(89)81605-9
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发表时间：
1989
期刊：
影响因子：
3.5
通讯作者：
L. Mahan
中科院分区：
文献类型：
--
作者：
S. Lolait;A. O'Carroll;K. Kusano;L. Mahan
大鼠gaba受体的第三个β亚基的cDNA是用我们之前克隆的另一个β亚基作为探针克隆的[(1989)FEBS Lett. 246, 145-148]。该β-亚基（称为β2）的约8 kb cDNA编码一个由474个氨基酸残基组成的蛋白质，该蛋白质与大鼠和牛β1-和β3亚基具有约80%的序列一致性。克隆的β-亚基cDNA与大鼠gaba受体α - 1亚基cDNA在异种卵母细胞内共表达，产生具有gaba受体药理特性的功能受体和Cl -通道。与互换α-亚基相反[1988)Nature 335, 76-79]， α1/β受体复合物中β2-或β3亚基的交换不会显著改变表达受体的药理特性。人工合成亚基特异性寡脱氧核苷酸探针原位杂交组织化学显示，α1-、β2-和β3亚基mrna在大鼠中枢神经系统中有区域特异性表达。这些观察结果为gaba受体复合物的功能异质性提供了额外的分子基础。
The cDNA for a third β-subunit of the rat GABAareceptor has been cloned using another β-subunit, which we had previously cloned [(1989) FEBS Lett. 246, 145-148], as a probe. The ~ 8-kb cDNA for this β-subunit (termed β2) encodes a protein of 474 amino acid residues that shares ~ 80% sequence identity with the rat and bovine β1- and β3-subunits. Coexpression of the cloned β-subunit cDNA with the α1-subunit cDNA of the rat GABAareceptor inXenopusoocytes produced a functional receptor and Cl−channel with pharmacological characteristics of a GABAareceptor. In contrast to interchanging α-subunits [(1988) Nature 335, 76-79], exchange of β2- or β3-subunits in α1/β receptor complex did not markedly alter the pharmacological properties of expressed receptors. In situ hybridization histochemistry with synthetic subunit-specific oligodeoxynucleotide probes revealed a region-specific expression of α1-, β2- and β3-subunit mRNAs in the rat central nervous system. These observations provide an additional molecular basis for the functional heterogeneity in the GABAAreceptor complex.
DOI：
10.1073/pnas.84.14.4767
发表时间：
1987-07-01
影响因子：
11.1
作者：
TABOR, S;RICHARDSON, CC
通讯作者：
RICHARDSON, CC
苯二氮卓受体与 γ-氨基丁酸 (GABA) 受体亚群偶联：来自定量放射自显影研究的证据。
DOI：
--
发表时间：
1981
期刊：
The Journal of pharmacology and experimental therapeutics
影响因子：
--
作者：
Unnerstall,JR;Kuhar,MJ;Niehoff,DL;Palacios,JM
通讯作者：
Palacios,JM