The utility of mitochondrial DNA fragments for genetic identification of forensically important sarcophagid flies (Diptera: Sarcophagidae) in China.
The utility of mitochondrial DNA fragments for genetic identification of forensically important sarcophagid flies (Diptera: Sarcophagidae) in China.
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发表时间：
2012-03
影响因子：
0.8
通讯作者：
Yadong Guo;Jifeng Cai;F. Xiong;H. Wang;J. Wen;J. B. Li;Y. Q. Chen
中科院分区：
文献类型：
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作者：
Yadong Guo;Jifeng Cai;F. Xiong;H. Wang;J. Wen;J. B. Li;Y. Q. Chen
肉蝇的物种诊断解剖学特征对于大多数未成熟阶段甚至成虫来说都是未知的，现有的关键可能是不完整的或非专家难以使用的。石棺与石棺的形态特征高度相似，极大地阻碍了石棺用于PMI估算。基于DNA的方法可以作为形态学方法的补充手段，用于鉴定具有重要法医学意义的石棺类苍蝇。然而，仅仅依靠单个DNA片段来界定物种被认为是不可靠的，特别是当片段很小时。给出了与身体相关的中国最重要的肉蝇类群的细胞色素氧化酶亚基2(COII)和16S核糖体RNA(16S RRNA)基因的部分区域的序列数据，为中国肉蝇科数据库的实施提供了参考。对测序片段的系统发育分析表明，所有石棺类标本被正确地归入5个种，这表明用较短的片段分离同属物种的可能性。
Species-diagnostic anatomical characters of fleshflies are not known for most immature stages or even adults, and an existing key may be incomplete or difûcult for nonspecialists to use. The use of sarcophagids for PMI estimations has been greatly hampered by their highly similar morphological characters. DNA-based method can be used as a supplemental means of morphological method in identification of forensically important sarcophagid flies. However, relying solely on single DNA fragment for delimiting species is considered to be unreliable, especially when the fragment was small. Sequence data of selected regions of the cytochrome oxidase subunit two (COII) and 16S ribosomal RNA (16S rRNA) genes of the most important Chinese fleshfly taxa associated with cadavers are presented, which can be instrumental for implementation of the Chinese Sarcophagidae database. Phylogenetic analysis of the sequenced segments showed that all sarcophagid specimens were properly assigned into five species, which indicated the possibility of separation congeneric species with the short fragments.