Expression of estrogen receptor coactivators in the rat uterus.
Expression of estrogen receptor coactivators in the rat uterus.
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大鼠子宫中雌激素受体共激活剂的表达。
DOI：
10.1095/biolreprod63.2.361
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发表时间：
2000
影响因子：
3.6
通讯作者：
Bigsby,RM
中科院分区：
文献类型：
--
作者：
Nephew,KP;Ray,S;Hlaing,M;Ahluwalia,A;Wu,SD;Long,X;Hyder,SM;Bigsby,RM
核受体辅激活因子以配体依赖的方式与雌激素受体（ER）和其他核受体结合，并且它们增强配体依赖的转录激活。本研究检测了大鼠子宫中基础辅激活因子的表达，以研究这些基因的表达是否受雌二醇-17 β或他莫昔芬的调控。将切除卵巢的成熟和未成熟大鼠注射雌二醇-17 β、他莫昔芬或载体（即，芝麻油）。收集子宫并使用北方印迹和原位杂交分析来分析共激活因子mRNA表达的变化。在对照子宫中观察到拮抗剂转换蛋白（SPA）、SRC-1、GRIP 1、RAC 3、RIP 140和p300 mRNA的组成型子宫mRNA表达，ER配体治疗未改变辅激活因子mRNA水平。这些数据表明，这些共激活基因的表达是不敏感的雌二醇或他莫昔芬在大鼠子宫。在成熟和未成熟大鼠的子宫切片中，没有细胞类型特异性表达模式;然而，与基质和子宫肌层相比，银颗粒在管腔和腺上皮细胞中更丰富，表明共激活因子mRNA水平在子宫腔室中各不相同。因此，据我们所知，我们第一次表明，有组成性表达的几个子宫核受体辅激活剂在生理环境中，仍然不敏感雌激素调节。此外，我们推测，在腺和腔上皮细胞中，辅激活因子表达的组成性水平较高可能与这些子宫腔室的激素反应性增加有关。
Nuclear receptor coactivators associate in a ligand-dependent manner with estrogen receptors (ER) and other nuclear receptors, and they enhance ligand-dependent transcriptional activation. This study examined basal coactivator expression in rat uterus to investigate if expression of these genes is regulated by estradiol-17β or tamoxifen. Ovariectomized mature and immature rats were injected with estradiol-17β, tamoxifen, or vehicle (i.e., sesame oil) alone. Uteri were collected and analyzed for changes in coactivator mRNA expression using Northern blot and in situ hybridization analyses. Constitutive uterine mRNA expression of switch protein for antagonist (SPA), SRC-1, GRIP1, RAC3, RIP140, and p300 mRNAs was observed in control uteri, and treatment with ER ligands did not alter coactivator mRNA levels. The data suggest that expression of these coactivator genes is not sensitive to estradiol or tamoxifen in the rat uterus. No cell type-specific pattern of expression was apparent in uterine sections from mature and immature rats; however, silver grains were more abundant in luminal and glandular epithelial cells compared with the stroma and myometrium, indicating that coactivator mRNA levels vary among the uterine compartments. Thus, to our knowledge, we show for the first time that there is constitutive expression of several uterine nuclear receptor coactivators in a physiological setting that remains insensitive to estrogenic regulation. Furthermore, we speculate that higher constitutive levels of coactivator expression in glandular and luminal epithelial cells may be associated with increased hormonal responsiveness by these uterine compartments.