Proteome Differences Between Male and Female Fetal Cells in Amniotic Fluid
Proteome Differences Between Male and Female Fetal Cells in Amniotic Fluid
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DOI：
10.1089/omi.2010.0145
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发表时间：
2013-01-01
影响因子：
3.3
通讯作者：
Chan, Hong-Lin
中科院分区：
文献类型：
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作者：
Chen, Chih-Ping;Lai, Tzu-Chia;Chan, Hong-Lin
在哺乳动物中，性发育受遗传和生殖调控。这个过程开始于染色体结构（XY或XX）的建立，然后是性别依赖基因的表达。为了阐明雄性和雌性间充质细胞的差异蛋白质谱，本研究采用蛋白质组学方法。在这里，我们利用蛋白质组学为基础的方法，包括2D-DIGE和MALDI-TOF MS分析，以获得差异表达的蛋白质之间的男性和女性卵巢癌细胞。用2D-DIGE技术分离蛋白质样品后，在来自三个独立批次的羊水的男性和女性羊膜细胞之间差异表达45种蛋白质，对应于28种独特的蛋白质。在所有这些独特的鉴定点中，其中5个（膜联蛋白A1，组织蛋白酶D，细胞骨架19，蛋白二硫键异构酶和波形蛋白）在至少两个独立的实验中表现出超过1.5倍的上调或下调。重要的是，所确定的蛋白质参与蛋白质降解和蛋白质折叠显示上调在男性生殖细胞，这意味着在性别发育过程中的蛋白质降解和蛋白质折叠的差异调节。总之，所鉴定的差异表达蛋白可用作性别发育的潜在标记。此外，已建立的蛋白质组学平台还可进一步用于发现胎儿产前遗传性疾病的潜在生物标志物。
In mammals, sex development is genetically and hormonally regulated. The process starts with the establishment of chromosomal structures (XY or XX), followed by the expression of sex-dependent genes. In order to elucidate the differential protein profiles between male and female amniocytes, a proteomic approach has been performed in this study. Here, we utilized a proteomics-based approach including 2D-DIGE and MALDI-TOF MS analysis to obtain differentially expressed proteins between male and female amniocytes. After resolving protein samples with 2D-DIGE technique, 45 proteins corresponding to 28 unique proteins were differentially expressed between male and female amninocytes from three independent batches of amniotic fluid. Of all of these unique identified spots, five of them (annexin A1, cathepsin D, cytoskeletal 19, protein disulfide-isomerase, and vimentin) exhibited more than 1.5-fold upregulation or downregulation in at least two independent experiments. Importantly, the identified proteins involved in protein degradation and protein folding display upregulated in male amniocytes, implying the differential regulations of protein degradation and protein folding during sex development. In conclusion, the identified differentially expressed proteins may be employed as potential signatures for the sex development. Moreover, the established proteomic platform might further utilize to discover the potential biomarkers for the prenatal genetic disorders in fetus.