A common protein binds to two silencers 5′ to the human β-globin gene
A common protein binds to two silencers 5′ to the human β-globin gene
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一种常见蛋白质与人类 β-珠蛋白基因 5 端的两个沉默子结合
DOI：
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发表时间：
1989
期刊：
影响因子：
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通讯作者：
A. Schechter
中科院分区：
文献类型：
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作者：
P. Berg;Donna M. Williams;R. Qian;R. Cohen;S. Cao;M. Mittelman;A. Schechter
人类珠蛋白基因在发育过程中表达的时间序列表明这些基因的精确调控。最近的研究已经确定了人类-珠蛋白基因内部或两侧的一些DNA序列，这些序列在其调控中很重要，并且已经确定了与这些序列结合的几种蛋白质。我们发现了两种将5'与人类-珠蛋白基因结合的蛋白质。其中一个蛋白质，我们称之为BP1，与两个序列结合，一个在相对于帽位-550和-527 bp之间，另一个在-302和-294 bp之间。另一种蛋白质BP2与-275到-263 bp之间的序列结合。BP1和BP2的结合位点位于两个区域，在使用人红白血病细胞系K562的瞬时表达试验中，这两个区域作为沉默子起作用。这些结果和本文提出的其他结果表明，BP1可能是一种抑制蛋白。负调控似乎是组织和发育特异性珠蛋白基因调控的重要组成部分。
The temporal sequence of expression of human globin genes during development suggests precise regulation of these genes. Recent studies have characterized a number of DNA sequences within or flanking the human beta-globin gene which are important in its regulation and several proteins which bind to these sequences have been identified. We have found two proteins which bind 5' to the human beta-globin gene. One of these proteins, which we designate BP1, binds to two sequences, one between -550 and -527 bp relative to the cap site, the other between -302 and -294 bp. A second protein, BP2, binds to sequences between -275 and -263 bp. The binding sites for both BP1 and BP2 are in two regions which function as silencers in a transient expression assay using the human erythroleukemia cell line K562. These results and others presented here suggest that BP1 may act as a repressor protein. Negative regulation seems to be an important component of tissue and developmental specific globin gene regulation.