Prevalence of Endosymbionts in Bemisia tabaci Populations and Their In Vivo Sensitivity to Antibiotics
Prevalence of Endosymbionts in Bemisia tabaci Populations and Their In Vivo Sensitivity to Antibiotics
复制标题
烟粉虱群体内共生菌的流行及其体内抗生素敏感性
DOI：
10.1007/s00284-010-9614-5
复制
发表时间：
2010-10-01
影响因子：
2.6
通讯作者：
Qiu, Bao-Li
中科院分区：
文献类型：
--
作者：
Ahmed, Muhammad Z.;Ren, Shun-xiang;Qiu, Bao-Li
烟粉虱内共生菌既有初级共生菌又有次级共生菌，不同B的内共生菌种类不同。烟粉虱生物型本研究确定了（1）中国两种入侵生物型（B和Q）和一种本土生物型（Cv）中初级内共生体Portiera aleyrodidarum和次级内共生体Arsenophonus和Wolbachia的流行率以及（2）三种抗生素的体内效应（四环素、氨苄青霉素三水合物和利福平）对抗内共生菌;如果抗生素基本上抑制了内共生体，那么它可以用于确定内共生体对B的影响。烟粉虱在3种生物型中均检测到粉虱和沃尔巴克氏体，而砷螨仅在Q型和Cv型中检测到。在三种生物型的所有测试个体中均发现了粉虱。在B、Cv和Q生物型中，沃尔巴克氏体的感染率分别为58%、68%和48%。Q型虫体的感染率为44%，而Cv型虫体的感染率仅为22%。抗生素未能从任何个体的B、Cv和Q生物型中消除粉虱，但消除了50%至80%的成虫B中的次级内共生体砷菌和沃尔巴克氏体。烟粉虱抗生素的作用取决于内共生菌的种类、抗生素种类、B种类。烟粉虱生物型，以及这些因素之间的各种相互作用。利福平对砷螨的杀灭效果优于氨苄西林和四环素，与B无关。烟粉虱生物型四环素对Cv型和Q型Wolbachia的灭活效果优于氨苄青霉素和利福平，而对B型Wolbachia的灭活效果优于氨苄青霉素和利福平。
Bemisia tabaci can harbor both primary and secondary endosymbionts, and the specific endosymbionts can differ among different B. tabaci biotypes. This study determined (1) the prevalence of the primary endosymbiont Portiera aleyrodidarum and secondary endosymbionts Arsenophonus and Wolbachia in two invasive biotypes (B and Q) and one indigenous biotype (Cv) in China and (2) the in vivo effect of three antibiotics (tetracycline, ampicillin trihydrate, and rifampicin) against the endosymbionts; if an antibiotic substantially inhibits an endosymbiont, it could be used to determine the effect of that endosymbiont on B. tabaci. P. aleyrodidarum and Wolbachia were detected in all the three biotypes, while Arsenophonus was found only in the Q and Cv biotypes. P. aleyrodidarum was found in all tested individuals of the three biotypes. Infection rates of Wolbachia in the B, Cv, and Q biotypes were 58, 68, and 48%, respectively. The infection rate of Arsenophonus was 44% in the Q biotype but only 22% in the Cv biotype. The antibiotics failed to eliminate P. aleyrodidarum from any individual of the B, Cv, and Q biotypes but eliminated the secondary endosymbionts, Arsenophonus and Wolbachia, from 50 to 80% of the adult B. tabaci. The effect of the antibiotics depended on the species of endosymbiont, the antibiotic, the B. tabaci biotype, and various interactions between these factors. When used against Arsenophonus, the efficiency of rifampicin was better than ampicillin and tetracycline, regardless of B. tabaci biotype. When inactivating Wolbachia in Cv and Q biotypes, the efficiency tetracycline was better than ampicillin and rifampicin, and while the efficiency of tetracycline was better than rifampicin and ampicillin when they were used against Wolbachia in B biotype.