Serum-mediated enhancement of ANF accumulation in the culture medium of cardiac atriocytes.
Serum-mediated enhancement of ANF accumulation in the culture medium of cardiac atriocytes.
复制标题
血清介导的心肌细胞培养基中 ANF 积累的增强。
DOI：
10.1016/0006-291x(86)91070-3
复制
发表时间：
1986
影响因子：
3.1
通讯作者：
Homcy,CJ
中科院分区：
文献类型：
--
作者：
Sylvestre,DL;Zisfein,JB;Graham,RM;Homcy,CJ
培养的心肌细胞为研究 ANF 生物合成和调节提供了一个有用的系统。先前已证明，该肌细胞模型中储存和释放的 ANF 的主要形式是 17kD 激素原 proANF。我们在此报告，通过放射免疫测定和 SDS-PAGE 对这些心肌细胞释放的内在标记的 ANF 进行定量，与单独存在确定的化学培养基时的 ANF 积累相比，添加血清可促进这些细胞培养基中 ANF 积累增加 5-6 倍。血清的刺激作用是立竿见影的，并以线性方式持续至少 120 分钟。血清的这种作用可以通过向培养基中添加白蛋白或其他蛋白质来重现，但不能通过改变渗透压来重现。这种现象是否代表 proANF 释放增强，或者继发于抑制 proANF 降解，尚未确定。
Cultured cardiac myocytes provide a useful system for investigating ANF biosynthesis and regulation. It has previously been demonstrated that the predominant form of ANF stored in and released from this myocyte model is the 17kD prohormone, proANF. We report here that as quantitated both by radioimmunoassay and by SDS-PAGE of intrinsically labelled ANF released from these cardiocytes, the addition of serum promotes a 5–6 fold increase in ANF accumulation in the medium of these cells as compared to ANF accumulation in the presence of a defined chemical medium alone. The stimulating effect of serum is immediate and persists in a linear manner for at least 120 minutes. This effect of serum can be reproduced by the addition of albumin or other proteins to the medium but not by alterations in osmolality. Whether this phenomenon represents enhanced release of proANF or is secondary to inhibition of proANF degradation has yet to be determined.