Development of a rapid and sensitive RT-PCR method for the detection of deformed wing virus, a pathogen of the honeybee (Apis mellifera)
Development of a rapid and sensitive RT-PCR method for the detection of deformed wing virus, a pathogen of the honeybee (Apis mellifera)
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DOI：
10.1016/j.tvjl.2004.01.004
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发表时间：
2005-01-01
影响因子：
2.2
通讯作者：
Genersch, E
中科院分区：
文献类型：
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作者：
Genersch, E
变形翅病毒（DWV）是一种蜜蜂病原体，通过外寄生螨狄斯瓦螨传播给幼虫。它是一种昆虫小核糖核酸样的正链RNA病毒，最近已经确定了DWV ssRNA基因组的完整核苷酸序列。感染DWV的幼虫发育成成虫，表现出羽化大小的减小和特征性形态畸形，或者在化蛹期间死亡（DeJong等人，1982年; Bowen-Walker等人，1999年）。蜜蜂畸形包括畸形附肢、短肢和变色（图1）。感染DWV的蜜蜂寿命也会缩短（Kovac和克赖尔桑，1988年）。DWV被认为是由V. destructor传播的最严重的继发病原体之一。DWV的二次感染增加了狄斯瓦螨的病理学，并在瓦螨属侵染过程中的菌落崩溃中起主要作用（Martin，2001）。到目前为止，DWV的鉴定一直基于传统方法，如电子显微镜和ELISA技术（Bowen-Walker et al.，1999; Nordstrom，2003）。由于没有可用的蜜蜂细胞系，因此无法使用培养方法。传统的技术敏感性和特异性低，从而阻碍了发病率的分子分析，
Deformed wing virus (DWV) is a honeybee pathogen, transmitted to larvae by the ectoparasitic mite Varroa destructor. It is an insect picorna-like, positive-stranded RNA virus and recently the complete nucleotide sequence of the ssRNA genome of DWV has been determined. Larvae infected with DWV either develop to adult bees showing a reduction in emergence size and characteristic morphological deformities, or die during pupation (DeJong et al., 1982; Bowen-Walker et al., 1999). Bee deformity includes malformed appendages, shortened abdomens, and miscolouring (Fig. 1). Bees infected with DWV also have a reduction in life span (Kovac and Crailsheim, 1988). DWV is considered one of the most serious secondary pathogens vectored by V. destructor. Secondary infections with DWV add to the pathology of V. destructor and play a major role in colony collapse in the course of Varroa infestation (Martin, 2001).Until now, identification of DWV has been based on traditional methods such as electron microscopy and ELISA techniques (Bowen-Walker et al., 1999; Nordstrom, 2003). Culture methods cannot be used since no honeybee cell lines are available. The traditional techniques are low in sensitivity and specificity, thus hampering molecular analysis of the incidence and