cDNA-AFLP reveals a striking overlap in race-specific resistance and wound response gene expression profiles
cDNA-AFLP reveals a striking overlap in race-specific resistance and wound response gene expression profiles
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DOI：
10.1105/tpc.12.6.963
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发表时间：
2000-06-01
期刊：
影响因子：
11.6
通讯作者：
Jones, JDG
中科院分区：
文献类型：
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作者：
Durrant, WE;Rowland, O;Jones, JDG
番茄Cf-9基因对表达Avr9基因的真菌病原菌黄枝孢杆菌具有抗性。利用cDNA扩增片段长度多态性分析，揭示了烟草细胞在Avr9和cf -9介导的防御反应中表达快速改变的转录本。在基因诱导过程中，使用二苯肼来消除活性氧的产生。在检查的30,000个片段中，290个显示丰度改变，其中263个是独立于活性氧诱导的。获得了13个ACRE （Avr9/Cf-9快速诱导）基因的cDNA克隆。在环己亚胺存在的情况下，发生了ACRE基因诱导。Avr9诱导叶片中ACRE基因的表达。令人惊讶的是，在其他胁迫下，叶片中也会迅速但短暂地诱导出ACRE基因。一些ACRE蛋白的氨基酸序列与已知的蛋白质序列同源，如乙烯反应元件结合蛋白转录因子、N抗性蛋白、钙结合蛋白、13-脂氧合酶和RING-HP锌指蛋白。ACRE基因的快速诱导表明它们在植物的防御反应中起着关键作用。
The tomato Cf-9 gene confers resistance to races of the fungal pathogen Cladosporium fulvum expressing the Avr9 gene. cDNA amplified fragment length polymorphism analysis was used to display transcripts whose expression is rapidly altered during the Avr9- and Cf-9-mediated defense response in tobacco cell cultures. Diphenyleneiodonium was used to abolish the production of active oxygen species during gene induction. Of 30,000 fragments inspected, 290 showed altered abundance, of which 263 were induced independently of active oxygen species. cDNA clones were obtained for 13 ACRE (for Avr9/Cf-9 rapidly elicited) genes. ACRE gene induction occurred in the presence of cycloheximide. Avr9 induced ACRE gene expression in leaves. Surprisingly, ACRE genes were also rapidly but transiently induced in leaves in response to other stresses. The amino acid sequences of some ACRE proteins are homologous to sequences of known proteins such as ethylene response element binding protein transcription factors, the N resistance protein, a calcium binding protein, 13-lipoxygenase, and a RING-HP zinc finger protein. Rapid induction of ACRE genes suggests that they play a pivotal role during plant defense responses.