EFFECT OF DIETARY INDOLE-3-CARBINOL ON INTESTINAL AND HEPATIC MONOOXYGENASE, GLUTATHIONE S-TRANSFERASE AND EPOXIDE HYDROLASE ACTIVITIES IN THE RAT
EFFECT OF DIETARY INDOLE-3-CARBINOL ON INTESTINAL AND HEPATIC MONOOXYGENASE, GLUTATHIONE S-TRANSFERASE AND EPOXIDE HYDROLASE ACTIVITIES IN THE RAT
复制标题
DOI：
10.1016/0278-6915(84)90147-9
复制
发表时间：
1984-01-01
影响因子：
4.3
通讯作者：
BJELDANES, LF
中科院分区：
文献类型：
--
作者：
BRADFIELD, CA;BJELDANES, LF
十字花科蔬菜和饮食中存在的某些天然化合物引起的抗癌作用被认为是诱导细胞膜结合的细胞色素p -448依赖的单加氧酶或诱导细胞质中存在的谷胱甘肽s -转移酶（GST）的结果。雄性Sprague-Dawley大鼠在净化饮食中添加50- 500ppm的吲哚-3-甲醇（I3C），这是一种存在于十字花科蔬菜中的化合物，或添加25%的抱子甘蓝（芸苔属），在净化饮食中平衡一周后，喂食10天。从肝脏和肠道粘膜制备细胞质和微粒体。在50 ppm（增加6.1倍）、125 ppm（增加11.8倍）、250 ppm（增加14.1倍）和500 ppm（增加20.2倍）和25%球芽甘蓝（增加3.6倍）条件下，I3C显著提高了肠道芳烃羟化酶（AHH）活性（P < 0.05）。I3C显著提高了肠道乙氧香豆素o -去乙基酶（ECD）活性，分别为50、125、250和500 ppm I3C组的4.6、8.7、9.3和11.2倍，豆芽组的3.2倍。肝脏AHH和ECD均未因任何饮食处理而显著升高。豆芽饲粮可使肝脏和肠道谷胱甘肽s转移酶（GST）活性分别提高1.9倍和1.6倍（P < 0.05），而500 ppm I3C对其影响不显著。豆芽日粮可使小肠微粒体环氧化物水解酶（EH）活性提高2.0倍，但I3C对其无影响。肝细胞色素P-450增加了1.3倍，而500 ppm的I3C只增加了1.1倍。I3C对肠道单加氧酶诱导的无影响水平估计在16-25 ppm之间，这支持了13C在实验动物喂食富含十字花科蔬菜的饲料时观察到的单加氧酶诱导的大部分原因的观点。甘蓝还含有其他化合物，这些化合物负责诱导GST和EH活性。
The anticarcinogenic effects elicited by cruciferous vegetables and certain naturally occurring compounds present in the diet were suggested to be a result of induction of membrane-bound cytochrome P-448-dependent monooxygenases or induction of glutathione S-tranferase (GST) species present in the cytosol. Male Sprague-Dawley rats were fed on purified diets supplemented with 50-500 ppm indole-3-carbinol (I3C), a compound present in cruciferous vegetables, or with 25% Brussels sprouts (Brassica oleracea) for 10 days after a 1 wk equilibration on a purified diet. Cytosolic and microsomal fractions were prepared from liver and intestinal mucosae. Intestinal aryl hydrocarbon hydroxylase (AHH) activity was increased significantly (P < 0.05) over basal levels by I3C at 50 ppm (a 6.1-fold increase), 125 ppm (11.8-fold), 250 ppm (14.1-fold) and 500 ppm (20.2-fold) and by 25% Brussels sprouts (3.6-fold). Intestinal ethoxycoumarin O-deethylase (ECD) activity was also significantly increased by I3C, the increases being 4.6-, 8.7-, 9.3- and 11.2-fold with 50, 125, 250 and 500 ppm I3C, respectively, and 3.2-fold with the sprouts diet. Hepatic AHH and ECD were not increased significantly by any dietary treatment. Hepatic and intestinal glutathione S-transferase (GST) activities were increased (P < 0.05) 1.9- and 1.6-fold, respectively, by the sprouts diet but were not significantly affected even by 500 ppm I3C. Microsomal epoxide hydrolase (EH) activity of the small intestine was increased 2.0-fold by the sprouts diet but was unaffected by I3C. Hepatic cytochrome P-450 was increased 1.3-fold by the sprouts diet although I3C at 500 ppm only produced a 1.1-fold increase. A no-effect-level for I3C on intestinal monooxygenase induction was estimated to be between 16-25 ppm, supporting the contention that 13C accounted for much of the monooxygenase induction observed when experimental animals were fed diets high in cruciferous vegetables. B. oleracea contained other compounds which were responsible for the induction of GST and EH activities.